高效液相色谱法测定化妆品中羟基喹啉

Determination of 8-hydroxyquinoline in cosmetics by HPLC

建立高效液相色谱法测定不同种类化妆品中羟基喹啉含量的方法。用甲醇溶解羟基喹啉,经SHISEIDO C18色谱柱(4.6 mm×250 mm,5μm)分离,流动相采用0.1%甲酸和甲醇以及0.1%磷酸和甲醇等度洗脱,在254 nm波长同时进行检测。与标准方法相比较,本研究中流动相以甲酸或磷酸代替癸烷磺酸钠,避免对照品色谱图出现羟基喹啉盐类峰;羟基喹啉在进样范围内线性关系良好,相关系数均大于0.999,4种不同基质低(80%)、中(100%)、高(120%)3个梯度加样回收率为96.9%~112.9%,RSD为0.20%~2.55%(n=9)。所建立方法简单、可靠,具有较高的灵敏度和重现性,可用于测定化妆品中羟基喹啉含量。

A method for the determination of 8-hydroxyquinoline in different types of cosmetics by high performance liquid chromatography(HPLC)was established.8-Hydroxyquinoline was dissolved in methanol,and then separated by SHISEIDO C18(4.6 mm×250 mm,5μm)column using 0.1%formic acid and methanol or 0.1%phosphoric acid and methanol as mobile phase with isocratic elution,with the detection wavelengths at 254 nm.Compared with the standard method,the mobile phase in this study used formic acid or phosphoric acid instead of sodium decane-1-sulfonate to avoid the peak of hydroxyquinoline salt in the chromatogram of the reference substance.The calibration curves show good linear relationship in the range of injection volume and the correlation coefficients are above 0.999.The average recoveries spiked at 80%,100%,120%are in the range of 96.9%-112.9%with RSD of 0.20%-2.55%(n=9).The established method is simple,reliable,and has high sensitivity and reproducibility.The method can be used for determing the content of 8-hydroxyquinoline in cosmetics.