芝麻素对氟暴露斑马鱼生长性能及肠组织形态学的影响

Effects of Sesamin on Growth Performance and Intestinal Tissue of Zebrafish Exposed to Fluoride

为探讨芝麻素对氟暴露斑马鱼生长性能及肠组织形态学的影响,本试验将720尾斑马鱼随机分为6组,分别为对照组(CK)、芝麻素Ⅰ组(1 g,S1)、芝麻素Ⅱ组(2 g,S2)、80 mg/L氟暴露组(F)、80 mg/L氟暴露+芝麻素Ⅰ组(1 g,FS1)、80 mg/L氟暴露+芝麻素Ⅱ组(2 g,FS2),每组3个重复,每个重复40尾。并在试验第45、90天测量各组斑马鱼体长、体重、增长率(LGR)、增重率(WGR)、特定生长率(SGR),组织学法观察各组斑马鱼的肠组织结构,用Imageproplus图像软件系统测量肠道绒毛高度。结果发现,第45天时,与CK组相比,F组斑马鱼体重显著下降(P<0.05),添加芝麻素(FS1和FS2组)对其有一定改善作用但差异不明显(P>0.05)。试验第90天时,与CK组相比,F组斑马鱼体重和体长显著下降(P<0.05),添加芝麻素(FS1和FS2组)可一定程度上改善这种负作用,但效果并不显著(P>0.05)。在45和90 d时,F组LGR、WGR、SGR较对照组均下降,而FS1和FS2组较F组呈上升趋势。斑马鱼肠道组织切片观察发现,芝麻素还可在一定程度上改善氟暴露对斑马鱼肠道组织造成的负面效应。综上所述,芝麻素可对氟致斑马鱼生长及肠道组织的毒性效应产生一定缓解作用。

In order to study the effects of sesamin on the growth performance and the morphology of intestinal tissues of zebrafish,720 zebrafish were randomly divided into 6 groups:Control group(CK),sesaminⅠgroup(1 g,S1),sasaminⅡgroup(2 g,S2),80 mg/L fluoride group(F),80 mg/L fluoride+sesaminⅠgroup(1 g,FS1),and 80 mg/L fluoride+sasaminⅡgroup(2 g,FS2),with 3 replicates per group and 40 zebrafish per replicate.The body length,body weight,LGR,WGR,and SGR were determined and the intestine structures of zebrafish were observed using the histological method,and the height of intestinal villi were measured using the Imageproplus at 45th and 90th day.The results showed that at 45 d,compared with the control group,the body weight of zebrafish in group F was significantly decreased(P<0.05),and sesamin treatment could alleviate the decrease of body weight,however the difference was not significant(P>0.05).At 90 d,the body weight and body length of zebrafish in group F were remarkably reduced compared with the control group(P<0.05),and sesamin treatment could relieve the negative effects caused by fluoride to some extent,but the difference was not significant(P>0.05).After exposure for 45 and 90 d,the LGR,WGR,and SGR of zebrafish in group F were decreased compared to the control group,while they showed an increasing trend in groups FS1 and FS2 compared with the group F.Sesamin treatment could alleviate the damage caused by fluoride in intestinal tissue of zebrafish according to the tissue section of gut.All above results indicated that sesamin could attenuate the toxic effects of fluoride on growth performance and the intestinal tissues in zebrafish.