核桃青皮水提物对鸡生长性能及禽流感和新城疫免疫效果的影响

Study on the Effect of Water Extract from Walnut Green Husks on Growth Performance and Immune Effect of Avian Influenza and Newcastle Disease in Chickens

试验旨在研究核桃青皮水取物对云南本地土杂鸡生长性能和禽流感、新城疫抗体水平的影响。选取120只28日龄本地土杂鸡,随机分为4个组(对照组、试验1组、试验2组、试验3组,分别在饮水中添加0、1%、3%、5%核桃青皮水提物),每组30只(公母各半),1只为1个重复。生产性能测定结果表明:65日龄时,各试验组平均体重均显著高于对照组(P<0.05),且试验3组的明显高于其他试验组(P<0.05);试验3组平均日增重(ADG)为62.89 g,明显高于其他各组(P<0.05),料重比(F/G)也明显低于对照组和其他试验组,分别降低了12.00%、13.39%、5.17%(P<0.05)。新城疫(ND)和禽流感(H7、H5)抗体水平的测定结果表明,免疫46 d后试验3组鸡新城疫抗体水平明显高于对照组和其他试验各组(P<0.05)。免疫48 d后试验2和3组鸡禽流感(H7)抗体水平显著高于对照组和试验1组(P<0.05)。试验2和3组禽流感(H5)抗体水平与对照组无明显差异(P>0.05)。试验结果表明,核桃青皮水提物可提高云南本地土杂鸡的生长性能和新城疫、禽流感(H7)抗体水平,且其效果随着核桃青皮水提物饮水浓度的增加而提高,综合来看以5%核桃青皮水提物效果最好。

The purpose of the experiment was to study the effects of water extracts from walnut husks on the growth performance and the levels of avian influenza and Newcastle disease antibodies in indigenous hybrid chickens in Yunnan province.120 local indigenous hybrid chickens at 28 days old were randomly divided into 4 groups,control group,test group 1,2 and 3,with the walnut husks water extract was respectively added to the drinking water at a concentration of 0,1%,3%,and 5%.30 birds in each group(half male and female).The results of growth performance showed that,at 65 days of age,the body weights of test groups were significantly higher than that of control group(P<0.05),and that in test group 3 was significantly higher than the other two test groups(P<0.05).The average daily gain(ADG)of test group 3 was 62.89 g,and was significantly higher than the control group and other test groups(P<0.05).F/G of test group 3 was lower than that of the control group and other test groups(P<0.05),which was decreased by 12.00%,13.39%,and 5.17%,respectively.The antibody levels of Newcastle disease(ND)and avian influenza(H7,H5)were measured after immunization.After 46 d of immunization,the level of ND antibody in test group 3 was significantly higher than that in the control group and other test groups(P<0.05).After 48 days of immunization,the levels of H7 antibodies in test 2 and 3 groups were significantly higher than those in control group and test 1 group(P<0.05).Compare to control group,the level of H5 antibody had no significant difference in test group 2 and 3(P>0.05).The results showed that the water extract of walnut green husks could improve the growth performance of and the levels of antibodies against Newcastle disease and avian influenza(H7)in Yunnan native hybrid chickens,and its effect increased with the increase of water concentration of walnut green husks water extract.In general,the supplementation of 5%walnut green husks water extract was the best.