1914名河北地区汉族人群HLA-DQB1基因多态性分析

Analysis of HLA-DQB1 Gene Polymorphism in 1914 Han Nationality Population in Hebei Province

目的探讨河北汉族人群人类白细胞抗原-DQB1(HLA-DQB1)基因型遗传特性.方法对1914名河北地区汉族人群献血者采用聚合酶链反应-基于测序的分型技术(PCR-SBT)进行HLA-DQB1位点高分辨基因分型,采用直接计数法计算等位基因频率,并与中国其他地区人群基因频率进行比较.结果1914名河北汉族献血者中共检出21个HLA-DQB1等位基因,其中频率大于0.0500的常见等位基因共6个,分别为DQB1^*03:01、DQB1^*03:03、DQB1^*06:02、DQB1^*02:02、DQB1^*06:01和DQB1^*05:01.结论河北地区汉族人群DQB1具有丰富的多态性,基因分布有明显的地区特性,了解河北汉族人群HLA-DQB1的分布状况,有利于帮助临床寻找HLA匹配的造血干细胞无关供者,同时为我国人群群体遗传学研究等提供有价值的背景资料.

Objective To investigate the genetic characteristics of human leukocyte antigen-DQB1(HLA-DQB1)genotype in Hebei Han nationality population.Methods HLA-DQB1 high-resolution genotyping was performed by polymerase chain reaction-sequencing based typing(PCR-SBT)in 1 914 Hebei Han nationality population.The frequency of allele was calculated directly and compared with other Regions of Han nationality populations in China.Results 21 HLA-DQB1 alleles were detected from 1 914 Han nationality blood donors in Hebei,among which 6 were common alleles with frequency greater than 0.0 5 0 0,which were DQB1^*0 3∶0 1、DQB1^*0 3∶0 3、DQB1^*0 6∶0 2、DQB1^*0 2∶0 2、DQB1^*0 6∶0 1、DQB1^*0 5∶0 1.Conclusion DQB1 in Hebei Han nationality population is rich in polymorphism,and the distribution of genes has obvious regional characteristics.The knowledge of the distribution of HLA-DQB1 in Hebei Han nationality population will help to find the HLA-matched unrelated Hematopoietic stem cell donors and provide valuable basic information for population genetics research in China.