酶免抗-HCV阳性标本中NAT法及胶体金法检测相关性分析

A Correlation Study of NAT and Colloidal Gold Method in Detection of ELISA Positive HCV Samples of Blood Donors

目的通过比较酶免法(ELISA)及胶体金法检测抗-HCV和核酸(NAT)检测HCV RNA,分析3种检测方法的实际效果,为临床输血安全提供依据.方法选取经2种ELISA法检测均为阳性的献血者标本,同时进行核酸和胶体金法检测,对结果进行分析.结果45例抗-HCV反应性标本,核酸检测阳性率为37.8%,胶体金法检测阳性率为77.8%.NAT显示,在酶免试剂1和试剂2的S/CO值均≤10.00时,核酸均未检出;在S/CO值均>10.00时,核酸检出率分别为70.8%和62.9%.胶体金法检测显示,在酶免1和2的S/CO值为1.00~5.00时,检出率分别为46.1%和40%;在S/CO值为5.01~10.00时,检出率均为75%;在S/CO值>10.00时,检出率分别为95.8%和77.8%.结论随着S/CO值升高,NAT和胶体金检测法的检出率也随之升高;核酸检测法无法替代ELISA法检测HCV,ELISA法检测抗-HCV联合核酸检测法检测HCV RNA检测可提高丙肝检出率.

Objective To compare the colloidal gold method with nucleic acid test(NAT)using ELISA positive HCV samples so as to observe the practical significance of the three methods in the safety ensurance of clinical blood transfusion.Methods The samples of blood donors were found to be positive for HCV by two ELISA methods and the results were analyzed by means of NAT and colloidal gold method.Results The NAT positive rate was 37%in 45 cases of anti-HCV positive sera,and the rate of colloid gold test was 77.8%%.No NAT positive samples were found in those with average value of S/CO below 10.0 when examined by two ELISA reagents whereas the frequencies of positive NAT was 70.8%and 62.9% in those with the average value of S/CO over 10.0.The Colloidal gold method revealed the positive rates of 46.1% and 40%respectively in those whith the value of S/CO between 1.0 and 5.0. The positive rates of colloidal gold method reached 75%in the samples of S/CO values between 5.0 and 10.0 and 95.8% in those of S/CO over 10.0. Conclusion The positive ratesof NAT and Colloidal gold method are elevated with increase of S/CO value tested by ELISAs. The NAT is irreplaceable forconventional ELISAs but combination of ELISAs with NAT might improve the detection of HCV RNAs.