猫4种不同来源间充质干细胞的生物学特性比较

Comparison of Biological Characteristics of Feline Mesenchymal Stem Cells Derived from Four Different Tissues

为比较猫的脂肪、骨髓、羊膜、脐带等器官组织4种不同器官来源间充质干细胞(Mesenchymal stem cells,MSC)的生物学特性,本研究通过全血培养法分离培养骨髓来源的间充质干细胞,并采用消化法分离培养脂肪、脐带和胎盘来源的间充质干细胞,比较它们的细胞形态、生长曲线、成脂成骨分化能力以及细胞表面标志物等生物学特性。结果显示,分离得到的4种不同来源的MSC均呈贴壁生长,细胞形态呈梭形、多棱形;均具有成脂成骨分化能力;脂肪源和骨髓源MSC的细胞增殖速度较快,细胞倍增时间较短,而羊膜与脐带源的MSC增殖能力较差,细胞倍增时间较长,4种细胞中脂肪来源MSC的增长活性最好,细胞传至第9代的时候依然保持良好的增殖活性,提示AD-MSC的临床应用可能比较好;4种MSC细胞表面均高表达CD105、CD90和CD44等标志物,低表达CD34。

To compare the biological characteristics of Mesenchymal Stem Cells(MSC)derived from adipose,bone marrow,amniotic membrane and umbilical cord,whole blood culture was used to isolate bone marrow-derived MSC and digestion to isolate others-derived MSCs.Then the morphology,growth characteristics,differentiation capacity,and cell surface markers were compared and analyzed.The results showed that feline MSC derived from 4 different tissues had common characteristics of adherent growth,spindle-shaped and polygonal-shaped.They all had the ability of adipogenic and osteogenic differentiation.The cell proliferation rate of adipose derived-MSC and bone marrow derived-MSC were faster than amniotic membrane derived-MSC and umbilical cord derived-MSC,and the doubling time of MSC from adipose and bone marrow was shorter.The adipose derived-MSC demonstrated the best growth activity and potential application prospect at clinic,because it maintained fine proliferative activity when they passed to the 9th generation.Cell surface markers of CD44,CD90 and CD105 were higher expressed,and CD34 were less expressed in four kinds of MSC.