SMBBR处理焦化废水性能及菌群结构响应关系

Performance and flora structure response relation in the SMBBR treatment of the coking wastewater

采用特异性移动床生物膜反应器(SMBBR)处理焦化废水,连续监测生物反应器处理性能.通过HS-GC/MS和Illumina高通量测序探究污染物降解与生物膜菌群结构的响应关系;利用CCA分析废水变量对微生物菌群结构的影响关系.结果表明,系统稳定运行50d时总酚去除率达96.62%,100d时硫氰化物和氰化物完全降解,其中酚、硫氰化物和氰化物对NH4^+-N的降解具有毒性抑制作用.HS-GC/MS结果显示,经过好氧处理后,80%以上的有机物被完全去除,其中包括全部酚、部分含N、O杂环化合物和长链烷烃等.测序结果表明,反应时间的不同,生物膜菌群丰度和多样性存在差异.反应期间Proteobacteria(变形菌门)相对丰度最高(20.57%~34.55%),促进了苯酚的降解;优势菌属为norank_f_ODP1230B8.23、unclassified_o_Micrococcales、norank_f_Anaerolineaceae;此外,Thauera(陶厄氏菌属)、Ottowia、unclassified_o_Rhizobiales(根瘤菌属)和Thiobacillus(硫杆菌属)为系统中苯酚、SCN-和CN-的降解优势菌.CCA分析表明,pH值与Nitrospira(硝化菌属)正相关性最大,有效控制pH值可有助于硝化反应的稳定运行.本文研究结论可为生物膜法处理焦化废水提供理论依据.

In this paper,a specific moving bed biofilm reactor(SMBBR),was engaged to treat the coking wastewater with its treatment performance being monitored continuously.The response relation between pollutants degradation and biofilm flora structure was illustrated by HS-GC/MS and Illumina high-throughput sequencing,and the influence of wastewater variables on microbial flora structure was analyzed by CCA.Results showed that the total phenol removal rate was 96.62percent when the system was operated stably for 50days,the thiocyanide and cyanide were completely degraded at 100days,phenol,thiocyanide and cyanide had toxic inhibition on NH4^+-N degradation.HS-GC/MS results exhibited that after the aerobic treatment more than 80percent of the organic pollutants,including all phenols,partially the N,O heterocyclic compounds and long-chain alkanes were removed.The sequencing results displayed that there existed difference in the biofilm flora abundance and diversity based on the change of the reaction time.Proteobacteria,which promotes the degradation of phenol,had the highest relative abundance during the reaction.The dominant genera were norank_f_ODP1230B8.23,unclassified_o_Micrococcales,and norank_f_Anaerolineaceae.In addition,Thauera,Ottowia,unclassified_o_Rhizobiales and Thiobacillus proved to be the dominant degrading bacterium of phenol,SCN-and CN-in the system.CCA analysis exhibited that the pH value is the most positively correlated with Nitrospira,and effective pH control can contribute to the stable operation of the nitrification reaction.All these provide a theoretical basis for the biofilm treatment of the coking wastewater.