摘要
[Objectives]The effect and mechanism of grape seed and sweet wormwood extract on growth of Monopterus albus were studied.[Methods]1 500 healthy M.albus with average mass of 23.8 g/tail were randomly divided into 5 groups.The control group was fed with basal diet and the experimental groups were fed with 10,20,30 and 40 g/kg of grape seed and sweet wormwood extract,respectively.The growth performance of the fish was measured after 60 d of feeding at 23-28℃.[Results]The addition of grape seed and sweet wormwood extract in basal diet could increase the body weight gain rate,specific growth rate and protein efficiency,and decrease the feed coefficient.The activity of digestive enzymes,glutamic-oxaloacetic transaminase,glutamic-pyruvic transaminase,blood alkaline phosphatase and serum total protein was increased,and the contents of blood glucose and triglyceride were decreased.Compared with the control group,the above indexes showed significant difference(P<0.05)when the dosage was 20 and 30 g/kg(P<0.05).The difference was not significant(P>0.05)when the dosage was 10 and 40 g/kg(P>0.05).[Conclusions]As a feed additive,the plant extract from grape seed and sweet wormwood could increase digestive enzyme activity and regulate blood biochemical parameters of fish body.It had the function of promoting the digestion and absorption,enhancing the metabolic activity of 3 main nutrients,and hastening the growth of fish.
[Objectives] The effect and mechanism of grape seed and sweet wormwood extract on growth of Monopterus albus were studied. [Methods] 1 500 healthy M. albus with average mass of 23.8 g/tail were randomly divided into 5 groups. The control group was fed with basal diet and the experimental groups were fed with 10, 20, 30 and 40 g/kg of grape seed and sweet wormwood extract, respectively. The growth performance of the fish was measured after 60 d of feeding at 23-28 ℃. [Results] The addition of grape seed and sweet wormwood extract in basal diet could increase the body weight gain rate, specific growth rate and protein efficiency, and decrease the feed coefficient. The activity of digestive enzymes, glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase, blood alkaline phosphatase and serum total protein was increased, and the contents of blood glucose and triglyceride were decreased. Compared with the control group, the above indexes showed significant difference(P<0.05) when the dosage was 20 and 30 g/kg(P<0.05). The difference was not significant(P>0.05) when the dosage was 10 and 40 g/kg(P>0.05). [Conclusions] As a feed additive, the plant extract from grape seed and sweet wormwood could increase digestive enzyme activity and regulate blood biochemical parameters of fish body. It had the function of promoting the digestion and absorption, enhancing the metabolic activity of 3 main nutrients, and hastening the growth of fish.
基金
Supported by Hunan Science and Technology Department Planning Project(2012NK3056)
