摘要
This study was conducted to investigate the effects of different concentrations ofβ-mercaptoethanol on the development ability of oocytes in Dorper sheep at the age of 6 weeks.In this study,6 Dorper sheep of 6 weeks old were chosen to induce oocyte collection in vivo by gonadotropin,and A and B grade cumulus oocyte complexes were obtained(COCs)with different concentrations of(0,50,70 and 100μmol/L)ofβ-mercaptoethanol in the mature liquid.The cleavage rate and blastocyst rate were counted after matured oocytes and capacitated sperm were co-incubated and fertilized eggs were cultivated.The results showed that compared with the cleavage rate(59.13%)and the blastocyst rate(12.17%)of the control group,the cleavage rates(60.87%,63.48%and 65.22%)and blastocyst rates(14.78%,17.39%and 21.74%)of the test groups I,II and III were higher(P>0.05),and the order was test group III>test group II>test group I>control group.The cleavage rate and blastocyst rate of the 100μmol/Lβ-mercaptoethanol group increased by 6.09 percentage points and 9.57 percentage points,respectively.It indicated that the addition of a certain amount ofβ-mercaptoethanol in the mature liquid could improve the developmental ability of oocytes and the quality of fertilized eggs of the 6-week-old Doper sheep.When theβ-mercaptoethanol concentration was within 100μmol/L,the cleavage rate and the blastocyst rate of oocytes were on the rise.The optimal concentration ofβ-mercaptoethanol in the mature liquid still needs further study.
This study was conducted to investigate the effects of different concentrations of β-mercaptoethanol on the development ability of oocytes in Dorper sheep at the age of 6 weeks. In this study, 6 Dorper sheep of 6 weeks old were chosen to induce oocyte collection in vivo by gonadotropin, and A and B grade cumulus oocyte complexes were obtained(COCs) with different concentrations of(0, 50, 70 and 100 μmol/L) of β-mercaptoethanol in the mature liquid. The cleavage rate and blastocyst rate were counted after matured oocytes and capacitated sperm were co-incubated and fertilized eggs were cultivated. The results showed that compared with the cleavage rate(59.13%) and the blastocyst rate(12.17%) of the control group, the cleavage rates(60.87%, 63.48% and 65.22%) and blastocyst rates(14.78%, 17.39% and 21.74%) of the test groups I, II and III were higher(P>0.05), and the order was test group III>test group II>test group I>control group. The cleavage rate and blastocyst rate of the 100 μmol/L β-mercaptoethanol group increased by 6.09 percentage points and 9.57 percentage points, respectively. It indicated that the addition of a certain amount of β-mercaptoethanol in the mature liquid could improve the developmental ability of oocytes and the quality of fertilized eggs of the 6-week-old Doper sheep. When the β-mercaptoethanol concentration was within 100 μmol/L, the cleavage rate and the blastocyst rate of oocytes were on the rise. The optimal concentration of β-mercaptoethanol in the mature liquid still needs further study.
基金
Supported by Earmarked Fund for Construction of National Wool Sheep Industry Technology Research System(CARS-39-24)
Fund for Science and Technology Innovation Team in Shanxi Province(201705D131028-20)
