人参皂苷对大鼠脑血管内皮细胞MEG3表达的干预作用及促进血管新生的研究

Research on the effects of ginsenoside on MEG3 expression and its effects on promoting angiogenesis in rat cerebral vascular endothelial cell

目的:观察人参皂苷(GS)对大鼠脑血管内皮细胞长链非编码RNA maternally expressed 3(MEG3)表达和血管新生的影响。方法:将大鼠脑微血管内皮细胞系(RBE4)培养在内皮细胞培养基中,分为Control组MEG3 siRNA组和GS组。实时荧光定量PCR法检测GS对RBE4的MEG3、血管内皮生长因子A(VEGFA)和血管内皮生长因子受体2(VEGFR2)基因表达量的影响。Western Blot检测GS对RBE4细胞VEGFA和VEGFR2蛋白表达量的影响。同时观察GS对RBE4细胞迁移和成管能力的影响。结果:与Control组比较,MEG3 siRNA组和GS组显著降低了MEG3的基因表达量(P<0.01),同时提高了VEGFA和VEGFR2的基因(P<0.01)和蛋白的表达量;RBE4的迁移能力和成管能力都显著增强(P<0.01,P<0.05)。结论:GS通过降低RBE4MEG3的表达量,从而上调VEGFA、VEGFR2的表达,并显著促进了血管新生。

Objective:To observe the effects of ginsenoside(GS)on the expression of long-chain non-coding RNA maternally expressed 3(MEG3)in rat cerebrovascular endothelial cells and its effect on angiogenesis.Methods:Rat brain microvascular endothelial cell line(RBE4)was cultured in endothelial cell culture medium and divided into the control group,MEG3 siRNA treatment group,and GS treatment group.Real-time fluorescence quantitative PCR was used to detect the effects of GS on the expression of MEG3,vascular endothelial growth factor A(VEGFA)and vascular endothelial growth factor receptor 2(VEGFR2)genes in RBE4.Western Blot was used to detect the effect of GS on the expression of RBE4,VEGFA and VEGFR2.Results:Compared with the control group,both MEG3 siRNA group and GS group reduced the MEG3 expression(P<0.01),and increased the gene and protein expression of VEGFA and VEGFR2(P<0.01).The migration ability of RBE4,the small and tubulogenic ability were significantly enhanced(P<0.01,P<0.05).Conclusion:GS can up-regulate the expression of VEGFA and VEGFR2 by reducing the expression of RBE4 MEG3,and significantly promote angiogenesis.