强直性脊柱炎患者外周血单核细胞亚型检测的临床意义

Clinical Significance of Detection of Peripheral Blood Monocyte Subtypes in Patients with Ankylosing Spondylitis

目的探讨强直性脊柱炎(AS)患者外周血单核细胞亚型检测的临床意义.方法应用流式细胞仪检测66例AS患者外周血单核细胞亚型百分率,并与40例正常对照组进行比较.应用ELISA法测定血浆4种细胞因子(TNF-α,IL-6,IL-18和IL-17)的含量,并分析AS患者单核细胞亚型百分率与细胞因子表达水平的相关性.结果AS组中间型单核细胞百分率明显高于对照组(P<0.01),经典型单核细胞百分率低于对照组(P<0.01),非经典型单核细胞百分率在AS组与对照组间无差异(P>0.05);AS组血浆4种细胞因子与对照组比较均升高(P<0.05),且与中间型单核细胞含量呈正相关.结论中间型单核细胞与AS的发生密切相关;检测AS患者外周血单核细胞亚型含量可为AS诊断提供新的实验指标.

Objective To discuss the clinical significance of detection of peripheral blood monocyte cell subtypes in ankylosing spondylitis(AS)patients.Method The percentage of peripheral blood monocyte subtypes of 66 AS patients was measured by flow cytometry and compared with 40 negative controls.The amount of four kinds of cytokines in plasma(TNF-a,IL-6,IL-18 and IL-17)was determined by ELISA.And the correlation between the percentage of monocyte subtypes and the cytokine expression level in AS patients was analyzed.Results The percentage of intermediate monocytes in AS group was significantly higher than that in the negative control group(P<0.01).The percentage of classical monocytes was lower than that in negative control group(P<0.01).There was no(significant)percentage difference of non-classical monocytes between the AS group and the negative control group(P>0.05).Compared with the negative control group,the four kinds of cytokines in plasma were all higher in AS group(P<0.05),and it is the positive correlation with the amount of intermediate monocytes.Conclusion Intermediate monocytes are closely related to the occurrence of AS.Detection of the amount of monocyte subtypes in peripheral blood of AS patients can provide new experimental indication for the AS diagnosis.