卵巢癌组织中CXCR4表达的临床意义及与β-catenin的相关性分析

Clinical significance of CXCR4 expression in ovarian cancer and its correlation with β-catenin

目的检测CXCR4在卵巢癌组织及细胞株中的表达,探讨其与临床病理特征之间的关系及与β-catenin表达的相关性。方法免疫组织化学方法检测CXCR4、β-catenin在7例正常卵巢上皮组织及49例卵巢癌组织中的表达,分析CXCR4与临床病理特征之间的关系,Pearson法分析CXCR4与β-catenin表达的相关性。通过real-time PCR方法检测CXCR4在正常卵巢上皮组织、卵巢癌组织及卵巢癌细胞株中的mRNA表达。通过Western blot方法检测CXCR4在正常卵巢上皮组织及卵巢癌细胞株中的蛋白表达。结果CXCR4与β-catenin在卵巢癌组织中均具有异常表达,两者之间具有显著的相关性(r=0.628,P<0.001)。CXCR4的表达与卵巢癌患者的淋巴结转移密切相关(P=0.016)。CXCR4 mRNA在卵巢癌组织及卵巢癌细胞株CAOV3、SKOV3和A2780中均具有高表达,其表达量分别是正常卵巢上皮组织中CXCR4 mRNA表达量的6.10±2.4倍、6.01±0.3倍、4.33±0.3倍和3.62±0.6倍,差异均具有统计学意义(P<0.001)。卵巢癌细胞株CAOV3、SKOV3和A2780中CXCR4蛋白表达是正常卵巢上皮组织中的3.51±0.08,2.95±0.14,2.82±0.10倍,差异均具有统计学意义(P<0.001)。结论CXCR4在卵巢癌组织及细胞株中均有高表达,与β-catenin表达显著相关,且与卵巢癌淋巴结转移密切相关。

Objective To investigate the expression of CXCR4 in malignant epithelial ovarian tumors and ovarian cancer cell lines,and investigate its relationship with clinicopathological features and the expression ofβ-catenin.Methods Immunohistochemical analysis was used to detect the expression of CXCR4 andβ-catenin in 49 ovarian cancer tissues and 7 normal ovarian tissues.The relationship between CXCR4 and clinicopathological features in ovarian cancer was analyzed.Pearson analysis was applied to study the correlation of CXCR4 andβ-catenin expression.Real-time PCR method was used to analyze the mRNA expression of CXCR4 in normal ovarian tissues,malignant epithelial ovarian tumors and ovarian cancer cell lines.Western blot was used to analyze the protein expression of CXCR4 in normal ovarian tissues and ovarian cancer cell lines.Results Abnormal expression of CXCR4 andβ-catenin was found in ovarian cancer tissues.There was significant correlation between CXCR4 andβ-catenin expression in ovarian cancer tissues(r=0.628,P<0.001).The expression of CXCR4 was correlated to the lymphatic metastasis(P=0.016).The mRNA expression levels of CXCR4 in ovarian cancer tissues and in ovarian cancer cell lines CAOV3,SKOV3 and A2780 were 6.10±2.4,6.01±0.3,4.33±0.3 and 3.62±0.6 times as much as that in normal ovarian epithelial tissues,and there was significant difference(P<0.001).The protein expression levels of CXCR4 in ovarian cancer cell lines CAOV3,SKOV3 and A2780 were 3.51±0.08,2.95±0.14 and 2.82±0.10 times as much as that in normal ovarian tissue,and there was significant difference(P<0.001).Conclusion CXCR4 is highly expressed in malignant ovarian tumors and ovarian cancer cell lines,and it is correlated to the lymphatic metastasis and expression ofβ-catenin.