循环增强荧光免疫技术与化学发光免疫法检测血浆BNP的比较研究

Comparative study on CEIFA and CLIA in determination of plasma BNP

目的分析和评价循环增强荧光免疫技术(cyclic enhanced immunofluorescent assay,CEIFA)与化学发光免疫法(chemiluminescent immunoassay,CLIA)在血浆B型脑钠肽(B-type natriuretic peptide,BNP)检测中的应用价值。方法采用CEIFA和CLIA法分别检测50例心内科患者血浆BNP的水平,对检测结果的相关性、回收率、精密度、样本稳定性进行评估。结果与CLIA法比较,CEIFA法回归方程为Y=0.975X+2.910(r=0.987,P<0.01),回收率为94.49%~100.53%,批内和批间变异系数(CV)分别为2.85%~6.66%和6.21%~12.52%,高值和中值样本结果的稳定性更高。结论CEIFA法检测BNP具有较好的性能,较CLIA法有一定的优势,可满足临床试验室需求。

Objective To analyze and evaluate the application value of cyclic enhanced immunofluorescent assay(CEIFA)and chemiluminescent immunoassay(CLIA)in detection of plasma B-type natriuretic peptide(BNP).Methods The plasma BNP levels were measured by CEIFA and CLIA in 50 patients with cardiology.The correlation,recovery rate,precision and sample stability of the results were evaluated.Results Compared with CLIA,the regression equation of CEIFA was Y=0.975X+2.910(r=0.987,P<0.01).The results of high and median samples were more stable.The recovery rate of CEIFA was 94.49%~100.53%,the intra-batch and inter-batch coefficient of variation(CV)were 2.85%~6.66%and 6.21%~12.52%.The stability of high value or median value samples was higher than that detected by CLIA.Conclusion CEIFA for plasma BNP detection has a good performance,and has some advantages compared with CLIA,which can meet the needs of clinical laboratories.