仔猪副伤寒活菌计数参考疫苗保存期试验

Storage life test on live bacteria count reference of paratyphus vaccine for piglets

为了解仔猪副伤寒活菌计数参考疫苗候选物在18个月内的稳定性,对其活菌数用协作标定的方法进行统计,结果表明,仔猪副伤寒参考疫苗候选物在2℃~10℃冷藏条件下运输48 h,活菌数无明显下降,说明运输稳定性良好,随机抽取10瓶参考疫苗候选物进行均一性检验,菌数的变异系数小于10%,说明参考疫苗的均一性良好,协作标定统计的活菌数中位数为3.5×10^9 CFU/头份。参考疫苗候选物在18个月的保存期内菌数中位数为3.4~3.75×10^9 CFU/头份,未随保存时间的延长而下降,证明在18个月保存期内稳定性良好。本研究为仔猪副伤寒活菌计数参考疫苗的研究提供依据。

In order to study the stability of the paratyphus vaccine for piglet reference candidates in 18 months,the living bacterium number was statistically calculated in the same way to Collaboration calibration.The results showed that the bacterium number did not drop obviously when transported 48 hours under the condition of 2~10℃,showing a good transport stability.10 samples of reference vaccine candidates randoma were taken and homogeneity test was carried out.The coefficient of variation of the 10 samples was less than 10%,showing a good uniformity.The median value of the collaborative calibration statistics was 3.5×10^9 CFU/dose。Bacterium number of reference vaccine candidates was not decreased during the 18-month of the preservation period,with the median value from 3.4×10^9 CFU/dose to 3.75×10^9 CFU/dose,proving that the reference vaccine was stable in 18 months.This study provides a basis for the study of reference vaccine for paratyphoid of piglets.