1-MCP对冷胁迫‘香蕉李’磷脂组分和冷害影响研究

Effects of 1-MCP on Phospholipid Components and Chilling Injury of ’Banana Plums’ under Cold Stress

目的研究1-MCP处理对冷胁迫‘香蕉李’磷脂组分、相关酶活性和衡量冷害发生相关指标的影响,以证明1-MCP处理可以有效地抑制‘香蕉李’冷害的发生。方法将‘香蕉李’果实进行冷胁迫处理(4℃),对比对照组(10℃)分析冷胁迫对各项指标产生的影响,并研究1-MCP处理对‘香蕉李’果实采后冷胁迫温度下(4℃)磷脂组分、相关酶活性和衡量冷害发生相关指标的影响。结果通过1-MCP处理能有效地抑制磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)和磷脂酰肌醇(PI)向溶血磷脂酰胆碱(LPC)、溶血磷脂酰乙醇胺(LPE)和溶血磷脂酰肌醇(LPI)水解,并且能够抑制单半乳糖二酰基甘油(MGDG)向双半乳糖二酰基甘油(DGDG)转化,从而判定1-MCP处理维持了细胞膜的稳定性。采用0.5μL/L的1-MCP处理还有效地抑制了信号分子磷脂酸(PA)含量的上升,从而延缓了‘香蕉李’膜脂过氧化的发生。1-MCP处理有效延缓了LOX酶活性、PLD酶活性、MDA含量增加的时间和速率,抑制了细胞膜的降解,保持了细胞膜的完整性,抑制了冷害指数的升高。结论贮运前用1-MCP处理能延缓和降低‘香蕉李’果实在低温贮运期间的冷害症状,保持果实细胞膜的完整性,从而推迟了‘香蕉李’冷害的发生时间,保证了果实品质。

The work aims to study the effects of 1-MCP treatment on the phospholipid components,related enzyme activities and related indexes of chilling injury of'banana plums'under cold stress,so as to prove that 1-MCP treatment can effectively inhibit chilling injury of'banana plums'.'Banana plums'were placed under cold stress(4℃).The effects of cold stress on various indexes were analyzed by comparing with the control group(10℃),and the effects of 1-MCP treatment on phospholipid components,related enzyme activity and related indexes of chilling injury of'banana plum'at postharvest cold stress temperature(4℃)were studied.1-MCP treatment could effectively inhibit phosphatidyl choline(PC)and phosphatidyl ethanolamine(PE)and phosphatidyl inositol(PI)from being hydrolyzed to lysophosphatidyl choline(LPC),lysophosphatidyl ethanolamine(LPE)and lysophosphatidyl inositol(LPI),and inhibit mono-galactosyl-diaclyglycerol(MGDG)from being converted to digalactosyl-diacylglycerol(DGDG),thus judging that 1-MCP treatment maintained the stability of cell membrane.In addition,the 0.5μ/L 1-MCP treatment also effectively inhibited the increase of the content of signal molecule phosphatidic acid(PA),thus delaying the occurrence of membrane lipid peroxidation of'banana plum'.1-MCP treatment effectively delayed the increase time and rate of LOX enzyme activity,PLD enzyme activity and MDA content,inhibited the degradation of cell membrane,and maintained the integrity of cell membrane and inhibited the increase of chilling injury index.1-MCP treatment before storage and transportation can delay and reduce chilling injury symptoms during low temperature storage and transportation of'banana plums',and maintain the integrity of fruit cell membrane,thus postponing chilling injury occurrence time of'banana plums'and ensuring fruit quality.