Wi-Fi对成骨细胞ROS的影响

The effects of Wi-Fi on ROS in osteoblasts

通过对近距离(3 cm)暴露于2.45 GHz Wi-Fi射频辐射的MC3TC-E1细胞的比吸收率(specific absorption rate,SAR)和温度变化进行时域有限差分(finite-difference time-domain,FDTD)法仿真,检测细胞活性氧(reactive oxygen species,ROS),探索体外培养的成骨细胞对2.45 GHz射频辐射能量的吸收和升温效应,及其非热效应对ROS的影响.研究结果表明:输出功率为500 mW 2.45 GHz Wi-Fi辐射90 min可使细胞平均温度升高0.5℃,升温最高点小于1℃;细胞培养皿总平均SAR值为0.835 6 W/kg,最大值为1.259 4 W/kg,最小值为0.553 7 W/kg;90 min 2.45 GHz Wi-Fi射频能量的非热效应可引起成骨细胞ROS的升高,ROS和SAR值正相关,Wi-Fi长时间辐射会影响骨的代谢.

The specific absorption rate(SAR)and temperature changes in MC3TC-E1 cells layer exposed to 2.45 GHz Wi-Fi irradiation in close range(3 cm)were simulated by finite-difference time-domain(FDTD)method.The cells reactive oxygen species(ROS)was detected.The energy absorption and heating of 2.45 GHz radio frequency radiation on osteoblasts in vitro,and its non-thermal effects on ROS in osteoblasts were analyzed.The results show that the average cell temperature is increased by 0.5℃and the highest temperature is increased by less than 1℃after 90-minute Wi-Fi irradiation at 500 mW 2.45 GHz;the average SAR value of cell culture dish is 0.835 6 W/kg,the maximum value is 1.259 4 W/kg,and the minimum value is 0.553 7 W/kg;Wi-Fi radiation increases cell ROS;ROS and SAR are positively correlated;the temperature in the sham group with increasing temperature of 1℃does not affect ROS statistically;the non-thermal effect of 2.45 GHz radio frequency induces the increase of ROS in osteoblasts.