硒代蛋氨酸对种公鸡精液品质及精浆抗氧化能力的影响

Effects of Selenomethionine on the Semen Quality and Antioxidant Capacity of Seminal Plasma of Breeding Cocks

[目的]探究日粮中添加硒代蛋氨酸对种公鸡的精液品质和精浆抗氧化能力的影响。[方法]试验选取120只健康、体况相近的378日龄罗曼褐蛋用种公鸡,随机分为2组,对照组饲喂基础日粮,处理组在基础日粮中添加硒水平为1 mg/kg的硒代蛋氨酸,预饲期7 d,正试期28 d,每周采集精液,进行精液品质以及精浆抗氧化能力的测定。[结果]与对照组相比,处理组种公鸡精液量差异不显著(P>0.05),处理组精子密度、精子活率和精子活力极显著提高(P<0.01);与对照组相比,处理组谷胱甘肽过氧化物酶活性和超氧阴离子清除自由基能力显著提高(P<0.05),总超氧化物歧化酶活性和羟自由基清除能力极显著提高(P<0.01),但丙二醛含量差异不显著(P>0.05)。[结论]日粮添加硒含量为1 mg/kg的硒代蛋氨酸可以显著提高精液品质和精浆抗氧化能力,为后续提高种公鸡繁殖性能的营养调控提供理论支撑。

[Objective]To investigate the effects of dietary selenomethionine supplementation on the semen quality and antioxidant capacity of seminal plasma of breeding cocks.[Method]120 healthy Roman brown breeding cocks at 378-day-age were selected and randomly divided into 2 groups.The breeding cocks in control group was fed with the basic diet,and the breeding cocks in experimental group was fed with the basic diet with adding selenomethionine with 1 mg/kg selenium.After pre-feeding 7 days,the semen was samples every week during the trial period of 28 days to determine the semen quality and antioxidant capacity of seminal plasma.[Result]There was no significant difference of semen volume between treatment group and control group(P>0.05).Sperm density,sperm viability and sperm motility in treatment group extremely significantly increased than those in control group(P<0.01).Compared with the control group,the activity of glutathione peroxidase and the scavenging capacity of free radical by superoxide anion significantly increased in treatment group(P<0.05),the activity of total superoxide dismutase and the scavenging capacity of hydroxyl radical extremely significantly increased in treatment group(P<0.01).There was no significant difference of malondialdehyde content between treatment group and control group(P>0.05).[Conclusion]Dietary supplementation of selenomethionine with 1 mg/kg seleniumcan could significantly improve the semen quality and antioxidant capacity of seminal plasma,which could provide theoretical support for nutritional regulation for the subsequent improvement of reproductive performance of breeding cocks.