慢性非细菌性前列腺炎大鼠腰6～骶1背神经节中蛋白激酶G及B型钠尿肽的表达水平

Expression levels of protein kinase G and B-type natriuretic peptide in lumbar vertebra 6 to sacral vertebra 1 dorsal ganglion of rats with chronic nonbacterial prostatitis

目的探讨慢性非细菌性前列腺炎(CNP)大鼠腰6~骶1背神经节中蛋白激酶G(PKG)、B型钠尿肽(BNP)的表达水平。方法(1)将120只大鼠随机分为实验组与对照组各60只,实验组大鼠给予前列腺腹叶注射完全弗氏佐剂0.1mL,对照组则予以注射等量生理盐水,均1次/d,持续14d,建模后按常规饲养。分别于模型建立后3d、7d、14d,每组各分离20只大鼠的腰6~骶1背神经节,检测其PKG、BNP蛋白及mRNA表达情况。(2)将另外40只大鼠分为研究组与参照组各20只,均给予前列腺腹叶注射完全弗氏佐剂0.1mL,1次/d,持续7d,建模同样按常规饲养。建模7d后,研究组大鼠神经鞘内注入50.0μL的BNP,参照组大鼠注入等量生理盐水。检测两组大鼠腰6~骶1背神经节中PKG蛋白表达情况。结果(1)实验组建模后3d、7d、14dPKG及BNPmRNA表达量均高于对照组建模后3d水平(均P<0.05);建模后3d、7d、14d实验组PKG及BNP蛋白表达量均高于对照组(均P<0.05)。(2)研究组腰6~骶1神经节中PKG蛋白表达水平高于参照组(P<0.05)。结论CNP大鼠腰6~骶1背神经节中PKG、BNP水平升高,两者可能参与CNP相关性疼痛的发生及发展。

Objective To investigate the expression levels of protein kinase G(PKG)and B-type natriuretic peptide(BNP)in lumbar vertebra 6 to sacral vertebra 1(L6-S1)dorsal ganglion of rats with chronic nonbacterial prostatitis(CNP).Methods(1)A total of 120 rats were randomly divided into experimental group(n=60)and control group(n=60).Rats in the experimental group were injected with 0.1 mL complete Freund′s adjuvant once daily in the ventral lobe of prostate for 14 consecutive days,while rats in the control group with the same amount of normal saline once daily for 14 consecutive days,receiving routine feeding after modeling.L 6-S 1 dorsal ganglion was isolated from 20 rats in each group 3,7 and 14 days after modeling,respectively,to detect the expression of PKG and BNP proteins and mRNAs.(2)Another 40 rats were divided into study group(n=20)and reference group(n=20),all rats were injected with 0.1 mL complete Freund′s adjuvant in the ventral lobe of prostate once daily for 7 consecutive days,receiving routine feeding after modeling.Seven days after modeling,50.0μL BNP was injected into the nerve sheath of rats in the study group,and the same amount of normal saline was injected into the rats in the reference group.The expression of PKG protein in rat L6-S1 dorsal ganglion was detected in the two groups.Results(1)The expression of PKG and BNP mRNAs 3,7 and 14 days after modeling in the experimental group was higher than the expression 3 days after modeling in the control group(all P<0.05);three,7 and 14 days after modeling,the expression of PKG and BNP proteins in the experimental group was higher than that in the control group(all P<0.05).(2)The study group had a higher expression level of PKG protein in L6-S1 ganglion than the reference group(P<0.05).Conclusion PKG and BNP levels in L6-S1 dorsal ganglion exhibit an increase among rats with CNP,both may be involved in the occurrence and development of CNP-related pain.