阿魏酸钠对肠缺血再灌注大鼠肝肾功能及CRP、IL-6、IL-10水平的影响

Effects of sodium ferulate on liver and kidney function and levels of CRP, IL-6 and IL-10 in rats with intestinal ischemia-reperfusion

目的:探讨阿魏酸钠预处理对小肠缺血再灌注大鼠肝肾功能的影响。方法:健康雄性Wistar大鼠24只,制备肠缺血再灌注损伤模型(夹闭肠系膜上动脉45 mins,再灌注120 mins)16只,分为模型组和治疗组,余8只为假手术组。治疗组于术前1周每天给予1次阿魏酸钠110 mg/kg灌胃;假手术组仅分离肠系膜上动脉不夹闭。各组再灌注120 mins后取血测定肝肾功能[谷丙转氨酶(Alanine Aminotransferase,ALT)、谷草转氨酶(Aspartate Aminotransferase,AST)、尿素氮(Blood Urea Nitrogen,BUN)、血清肌酐(Creatinine,Cre)]及血清C反应蛋白(C-reactive Protein,CRP)、白介素-6(Interleukin-6,IL-6)、白介素-10(Interleukin-10,IL-10)水平。结果:模型组肝肾功能相关指标及血清CRP、IL-6、IL-10水平明显高于假手术组(P<0.05或0.01),治疗组肝肾功能相关指标及CRP、IL-6、IL-10水平明显低于模型组(P<0.05或0.01)。结论:阿魏酸钠对大鼠肠缺血再灌注后肝肾功能有保护作用,其机制可能与抑制CRP、IL-6、IL-10等炎性因子释放有关。

Objective:To investigate the effects of sodium ferulate pretreatment on liver and kidney function in rats with small intestinal ischemia-reperfusion.Methods:24 healthy male Wistar rats were randomly divided into a model group and a treatment group,and the remaining 8 rats were sham-operated group.The treatment group was given 110 mg/kg of sodium ferulate,once a day,for 1 week before surgery.The sham operation group only was separated the superior mesenteric artery without clipping.Blood samples were taken for 120 mins after reperfusion,and liver and kidney function and serum CRP,IL-6 and IL-10 levels were measured.Results:The indexes of liver and kidney function and serum CRP,IL-6 and IL-10 levels in the model group were significantly higher than those in the sham operation group(P<0.05 or 0.01).Related indicators in liver and kidney function and CRP,IL-6 and IL-10 were better in the treatment group than the model group(P<0.05 or 0.01).Conclusion:Sodium ferulate has protective effects on liver and kidney function after intestinal ischemiareperfusion in rats,and its mechanism may be related to inhibition of release of inflammatory factors such as CRP,IL-6 and IL-10.