羊附红细胞体体外培养最佳条件的探索

Determination of Optimum in vitro Culture Conditions of Mycoplasma ovis

近几年,内蒙古自治区锡林郭勒盟多地出现了一种羊病,发病率和死亡率都较高,最终诊断为羊附红细胞体病。为给羊附红细胞体(Mycoplasma ovis,M.ovis)深入研究奠定基础,参考支原体培养基,进行配方改良,对M.ovis的最佳培养条件进行了初步研究。结果表明:应用实验室自制ZP-S-BS培养基,48 h更换半量培养基,37℃培养效果最佳。感染M.ovis红细胞用ZP-S-BS培养能维持长时间存活状态,洗脱的M.ovis用ZP-S-BS培养仍可连续培养260 d,且能缓慢增殖。

In recent years,the prevalence of an infectious disease characterized by high morbidity and mortality in sheep herds has been observed in Xilin Gol League of Inner Mongolia.This disease was finally diagnosed as eperythrozoonosis,a zoonosis caused by Mycoplasma ovis(M.ovis).In order to lay a foundation for the in-depth study on M.ovis,the optimum conditions for in vitro culture of M.ovis was determined,with emphasis on formula improvement of culture medium by referring the Mycoplasma culture medium.The best growth of M.ovis was observed in a self-prepared ZP-S-BS culture medium under 37℃with replacement of half fresh medium at 48 h.The erythrocytes infected with M.ovis could be long-term in vitro cultured with the ZP-S-BS culture medium.The eluted M.ovis was able to be continuously cultured in vitro up to 260 days in ZP-S-BS culture medium and propagate slowly.