吉马酮对支气管哮喘小鼠外周血T淋巴细胞 IL-6及TNF-α表达的影响

GEMALONE ON THE EXPRESSION OF PERIPHERAL BLOOD T LYMP- HOCYTES IL-6 AND TNF-α IN MICE WITH BRONCHIAL ASTHMA

目的探讨支气管哮喘的治疗方法。方法制作33只支气管哮喘实验小鼠模型作为观察组,另选择同时期培育的30只健康小鼠作为对照组。观察组给予吉马酮治疗,治疗前后检测其外周血T淋巴细胞、IL-6、TNF-α的表达水平,并与对照组比较。结果观察组给药前CD 4^+、CD 4^+/CD 8^+、TNF-α及IL-6高于对照组(t=3.15~13.77,P<0.05),CD 8^+水平低于对照组(t=2.12,P<0.05);给药后观察组CD 4^+、CD 4^+/CD 8^+、TNF-α及IL-6均有下降,CD 8^+水平增高,与对照组相比无统计学意义(P>0.05)。结论吉马酮能改善支气管哮喘小鼠外周血T淋巴细胞、IL-6、TNF-α的表达水平。

Objective To explore the treatment of bronchial asthma.Methods Thirty-three bronchial asthma experimental mice were used as the observation group.Thirty healthy mice that were cultured at the same time were selected as the control group.The observation group was treated with gemone.The expression levels of peripheral blood T lymphocytes,IL-6 and TNF-αwere measured before and after treatment,and compared with the control group.Results The CD 4^+,CD 4^+/CD 8^+,TNF-αand IL-6 levels in the observation group were higher than those in the control group(t=3.15~13.77,P<0.05),and the CD 8+level was lower than the control group(t=2.12,P<0.05).After administration,the CD 4^+,CD 4^+/CD 8^+,TNF-αand IL-6 levels were decreased in the observation group,and the CD 8^+level was increased,which was not statistically significant compared with the control group(P>0.05).Conclusion Gimarone can improve the expression of T lymphocytes,IL-6 and TNF-αin peripheral blood of mice with bronchial asthma.