癌易感性候选基因2在肝细胞癌中的表达及其对患者预后的影响

Expression of cancer susceptibility candidate 2 in hepatocellular carcinoma and its influence on the prognosis of the patients

目的探讨肝细胞癌(HCC)患者长链非编码RNA癌易感性候选基因2(CASC2)的表达水平与患者预后的关系。方法回顾分析首都医科大学宣武医院及首都医科大学附属北京佑安医院2007年9月至2014年1月129例HCC患者资料。其中男性80例,女性49例,年龄21~73岁。实时荧光定量PCR检测肝癌及癌旁正常组织CASC2相对表达,分析其与临床病理特征的关系。生存分析采用Kaplan-Meier法,组间比较用log-rank检验。单因素和多因素Cox回归分析患者预后影响因素。结果129例患者肝癌组织CASC2相对表达为(0.84±0.05),明显低于癌旁组织(3.35±0.11),差异有统计学意义(P<0.05)。129例HCC患者CASC2相对表达高低与肿瘤直径、肿瘤分化程度和肿瘤分期相关。以CACS2相对表达的均值0.84为界值,129例患者分为低表达组(n=72)和高表达组(n=57)。CACS2高表达组总生存率高于低表达组,差异有统计学意义(P<0.05)。多因素分析,肿瘤分化程度(HR=0.20,95%CI:0.05~0.59)、肿瘤分期(HR=1.71,95%CI:1.02~2.99)和CACS2表达(HR=0.51,95%CI:0.08~0.92)为预后影响因素。结论CACS2的低表达可能与HCC的发生发展有关。CACS2高表达是HCC患者预后的保护因素。

Objective To investigate the correlations between expression of CASC2 and hepatocellular carcinoma(HCC)prognosis.Methods A total of 129 patients including 80 males and 49 females with HCC were includedin this study,ranging from 21 to 73 years in Xuanwu Hospital of Capital Medical University and Beijing You’an Hospital were retrospectively analyzed from September 2007 to January 2014.Expression of CASC2 was assessed using reverse transcription quantitative-polymerase chain reaction in HCC tissue and the adjacent normal tissue.The correlations between CASC2 mRNA level and clinicopathological parameters was investigated.The relationship between the expression of CASC2 and the prognosis of patients with HCC was analyzed by Kaplan-Meier method.A log-rank analysis was performed to identify group differences.Univariate and multivariate Cox analysis were used to analyze the variables affecting the patient’s prognosis.Results In 129 HCC samples,the level of CASC2 expression(0.84±0.05)was lower than(3.35±0.11)adjacent normal tissue(P<0.05).There were significant differences between CASC2 expression and tumor size,histological differentiation,and tumor stage in 129 HCC speciments.The median expression level of CACS2 in HCC tissues,0.84-fold,was used as the cut-off value to divide the 129 patients into two groups:low-expression group(n=72)and high-expression group(n=57).Overall survival rate of HCC patients with high CACS2 expression was significantly higher than those of patients with low CACS2 expression(P<0.05).Multivariate analysis indicated that histological differentiation(HR=0.20,95%CI:0.05~0.59),tumor stage(HR=1.71,95%CI:1.02~2.99)and CACS2 expression(HR=0.51,95%CI:0.08~0.92)were an independent predictor of overall survival.Conclusion Low expression of CACS2 might be associated with the occurrence and development of HCC.