高脂血症食蟹猴模型的建立及分析

Establishment and Analysis of Hyperlipidemia Model in Cynomolgus Monkey

目的建立高脂血症食蟹猴模型。方法采用高脂膳食饲料诱导食蟹猴,分别在4、8、12、18个月检测血清总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、甘油三脂(TG)指标,对其血脂指标的变化趋势进行分析。结果模型组在饲喂高脂膳食饲料4个月后,TC、HDL、LDL与实验前相比差异极显著(P<0.01);TG在饲喂高脂膳食饲料8个月后明显升高,与实验前相比差异极显著(P<0.01);造模后,高脂饲料组与正常对照组比较,TC、HDL、LDL、TG均高于对照组,差异极显著(P<0.01)。结论在本实验中,模型组TC、HDL、LDL、TG均呈现递增趋势,尤其是TC、LDL明显高于食蟹猴正常参考值水平,说明模型组食蟹猴具有高胆固醇和高、低密度脂蛋白血症的特征,初步建立了食蟹猴高脂血症动物模型。

Objective To establish Cynomolgus monkey model of hyperlipidemia.Method Cynomolgus were feed by high fat diet,the levels of total cholesterol(TC),high-density lipoprotein(HDL),low density lipoprotein(LDL),triglyceride(TG)of the Cynomolgus monkey were determined at 4,8,12,18months for analyzing the trend of each paramaters above.Result When it was 4 months,the level of TC,HDL,LDL were higher significantly than that before feeding the high fat diet(P<0.01).When it was 8 months,the level of TG was higher sifnificantly than that before feeding the high diet(P<0.01).And the levels of TC,HDL,LDL and TG of model group were significantly different from that of the control group(P<0.01)at 8 months,18 months.At this experiment,TC,HDL,LDL,TG all showed a increase trend,expecially the levels of TC and LDL much higher than the normal levels of Cynomolgus monkey.Conclusion Cynomolgus monkey of the model group has the feature of high total cholesterol and high low-density lipoprotein cholesterol,the method to establish Cynomolgus monkey model of experimental hyperlipidemia was feasible.