菜用豌豆核心种质构建的关键策略

Key construction strategies for core germplasm collection in vegetable pea(Pisum sativum)

利用已搜集的180份菜用豌豆材料进行核心种质构建策略研究。分别对所有种质材料进行单株荚数、每荚粒数、荚长、荚宽、荚厚、百荚鲜质量、百粒鲜质量及产量等性状进行调查,结果表明,搜集的材料具有丰富的遗传多样性。利用上述数据,采用最小距离逐步取样(minimum distance stepwise sampling,LDSS)法,分别选择4种遗传距离、8种取样比例进行核心种质构建策略研究,并采用极差符合率(coincidence rate of range,CR)和变异系数变化率(variable rate of coefficient of variation,VR)2个参数对构建策略进行评价;同时,利用主成分分析法和聚类分析法对构建的核心种质代表性进行鉴定。结果表明,采用LDSS法构建菜用豌豆核心种质的最佳遗传距离为欧式距离,最佳取样比例为25%。该构建策略将为菜用豌豆核心种质构建与高效利用奠定基础。

Core germplasm construction is one of the important means to utilize and manage plant germplasm resources.In present study,180 germplasms of vegetable pea(Pisum sativum)were collected to study the construction strategy of core germplasm.The agronomic and yield characters were investigated,including the number of pod per plant,number of seed per pod,pod length,pod width,pod thickness,fresh mass of 100-pod,fresh mass of 100-seed,and yield.The results revealed an abundant genetic diversity in these vegetable pea germplasms.Using these data,the construction strategies of core germplasm were studied by using the minimum distance stepwise sampling(LDSS)method with four genetic distances and eight sampling ratios.Then,the construction strategies were evaluated by using the coincidence rate of range(CR)and variable rate of coefficient of variation(VR).The principal component analysis and cluster analysis were conducted to validate the representativeness of core germplasms.The results showed that Euclidean distance(ED)combining 25%sampling ratio was the best construction strategy in core germplasm construction of vegetable pea based on LDSS method.This study will lay a foundation for the core germplasm construction and efficient utilization of vegetable pea.