利用TCGA数据集分析LncRNA LINC00319在肺腺癌中的表达及临床意义

Analysis of the expression and clinical significance of LncRNA LINC00319 expression in lung adenoarinoma utilizing TCGA datasets

目的研究长链非编码RNA(lncRNA)LINC00319在肺腺癌及其癌旁组织中的表达及临床意义。方法从癌症基因组图谱(TCGA)数据库下载516例肺腺癌组织(肿瘤组)与59例癌旁组织(癌旁对照组)RNAseq数据及对应的肺腺癌患者临床数据,对数据中LINC00319基因表达进行分析处理。分析比较其表达与肺腺癌临床病理特征的相关性及对预后的影响。通过COX风险比例模型评估肺腺癌患者预后的独立危险因素。采用基因集富集分析(GSEA)方法预测LINC00319功能富集的分子通路。结果LINC00319在肿瘤组中的表达水平显著高于癌旁对照组(P<0.05),其表达水平与性别、年龄、N分期无关(P>0.05),与吸烟、T分期、M分期以及Stage分期显著相关(P<0.05),生存分析显示肺腺癌患者中高LINC00319表达水平预示预后差(P<0.05)。多因素COX风险比例模型结果显示,高表达水平、Ⅲ+Ⅳ及T3+T4是肺腺癌患者预后差的独立危险因素(P<0.05)。在GSEA分析中,发现LINC00319功能主要富集在细胞黏附、细胞因子相互作用、血管内皮生长因子信号通路、细胞凋亡等通路中。结论LINC00319可能通过多种途径促进肺腺癌的发生、发展,进而影响肺腺癌患者的预后生存,因此,LINC00319可作为肺腺癌患者的预后标志物或潜在治疗靶点。

Objective To investigate the expression and clinical significance of long non-coding RNA(lncRNA)LINC00319 in lung adenocarcinoma and its adjacent tissues.Methods The RNAseq data of 516 cases of lung adenocarcinoma tissues(tumor group)and 59 cases of paracancer tissues(para-cancer control group)and the corresponding clinical data of lung adenocarcinoma patients were downloaded from the cancer genome atlas(TCGA)database,and the expression of LINC00319 gene in the data set was analyzed and processed.The correlation between its expression and the clinicopathological features of lung adenocarcinoma and its effect on prognosis were analyzed and compared.Independent risk factors for prognosis of patients with lung adenocarcinoma were assessed by COX risk ratio model.Gene set enrichment analysis(GSEA)was used to predict the functional enrichment of LINC00319 molecular pathways.Results The expression level of LINC00319 in tumor group was significantly higher than that in the para-carcinoma control group(P<0.05).The expression level had no correlation with gender,age and N-staging(P>0.05),and significantly correlated with smoking,T-staging,M-staging and Stage grading(P<0.05).Survival analysis showed that high LINC00319 expression predicted worse prognosis in lung adenocarcinoma patients(P<0.05).The results of multiariable COX risk scale model showed that,high expression level,stageⅢ+Ⅳand T3+T4 were independent risk factors for poor prognosis of patients with lung adenocarcinoma(P<0.05).In GSEA analysis,it was found that LINC00319 mainly enriched in cell adhesion,cytokine interaction,vascular endothelial growth factor signaling pathway,apoptosis and other pathways.Conclusion LINC00319 may promote the occurrence and development of lung adenocarcinoma through various ways,thereby affecting the prognosis and survival of lung adenocarcinoma patients.Therefore,LINC00319 can be used as a prognostic marker or potential therapeutic target for lung adenocarcinoma patients.