外周血ADAM8、IRF1表达水平与支气管哮喘患儿肺功能的相关性研究

Correlation study of expression of ADAM8, IRF1 in peripheral blood and pulmonary function in children with bronchial asthma

目的研究外周血去整合素金属蛋白酶8(ADAM8)、干扰素调控因子1(IRF1)的表达水平与支气管哮喘(BA)患儿肺功能的相关性。方法选择2018年1~8月在海口市妇女儿童医院(以下简称“我院”)接受治疗的84例BA患儿作为观察组,根据病情严重程度将其分为轻度组(30例)、中度组(44例)、重度组(10例)。另选同期在我院进行体检的健康儿童80名作为对照组。检测并比较各组肺功能指标及外周血ADAM8、IRF1的mRNA及蛋白表达水平,分析观察组患儿外周血ADAM8、IRF1的蛋白表达与其肺功能指标的相关性。结果观察组及轻、中、重度组外周血ADAM8、IRF1的mRNA及蛋白表达水平均明显高于对照组,差异均有高度统计学意义(P<0.01);重度组和中度组外周血ADAM8、IRF1的mRNA及蛋白表达水平明显高于轻度组,且重度组高于中度组,差异均有统计学意义(P<0.05)。观察组及轻、中、重度组患儿第1秒用力呼气容积(FEV1)、FEV1与用力肺活量的比值(FEV1/FVC)及呼气峰流速(PEF)均低于对照组,差异均有统计学意义(P<0.05);重度组和中度组FEV1、FEV1/FVC及PEF均明显低于轻度组,且重度组低于中度组,差异均有统计学意义(P<0.05)。Pearson相关性分析结果示,患儿外周血ADAM8、IRF1的蛋白表达与其FEV1、FEV1/FVC及PEF均呈负相关(rADAM8=-0.780、-0.773、-0.682,均P<0.01;rIRF1=-0.684、-0.710、-0.768,均P<0.01)。结论BA患儿外周血ADAM8和IRF1表达水平明显升高,且二者与肺功能具有密切关系,临床上可考虑将二者纳入BA患儿的监测靶点,从而辅助诊治过程。

Objective To study the correlation of the expression of deintegrin metalloproteinase 8(ADAM8),interferon regulatory factor 1(IRF1)in peripheral blood and pulmonary function in children with bronchial asthma(BA).Methods Eighty-four children with BA who were treated in Haikou Women&Children Hospital("our hospital"for short)from January to August 2018 were selected as observation group,and they were divided into mild group(30 cases),moderate group(44 cases)and severe group(10 cases)according to the severity of the disease.Eighty healthy children who underwent physical examination in our hospital at the same time were selected as control group.The pulmonary function indices,the expression levels of ADAM8,IRF1 mRNA and protein in peripheral blood of each group were detected and compared.The correlation between the expression of ADAM8,IRF1 protein in peripheral blood and pulmonary function indices was analyzed.Results The expression levels of ADAM8,IRF1 mRNA and protein in peripheral blood of observation group and mild,moderate,severe group were significantly higher than those of the control group,the differences were highly statistically significant(P<0.01);the expression levels of ADAM8,IRF1 mRNA and protein in peripheral blood of the severe group and moderate group were significantly higher than the mild group,and those of the severe group were significantly higher than the moderate group,the differences were statistically significant(P<0.05).The forced expiratory volume in 1 second(FEV1),FEV1/forced vital capacity(FEV1/FVC),peak expiratory flow(PEF)in the observation group and mild,moderate,severe group were significantly lower than the control group,the differences were statistically significant(P<0.05).The levels of FEV1,FEV1/FVC and PEF in the severe group and moderate group were significantly lower than the mild group,while the severe group was significantly lower than the moderate group,the differences were statistically significant(P<0.05).Pearson correlation analysis showed that,the expression of ADAM8,IRF1 protein was negatively correlated with FEV1,FEV1/FVC and PEF(rADAM8=-0.780,-0.773,-0.682,P<0.01;rIRF1=-0.684,-0.710,-0.768,P<0.01).Conclusion The expression of levels ADAM8 and IRF1 in peripheral blood of children with BA are significantly increased,and it is closely related to pulmonary function.It can be considered as the monitoring target of children with BA,in order to assist the diagnosis and treatment process.