水稻稻瘟病抗性基因Pik-2的遗传变异及进化分析

Genetic Variation and Evolution Analysis of Rice Blast Resistance Gene Pik-2

Pik-2位于水稻基因序列中第11号染色体,通过研究分析Pik-2位点在水稻基因遗传过程中的变异与进化,可以掌握水稻基因序列中第11号染色体的自然进化规律与恶性突变的诱发因素,从而制定相关的防治方案,提高水稻对稻瘟病的抗性。选择了400份非洲地区的水稻样本和400份亚洲地区的水稻样本,对所有水稻的第11号染色体Pik-2位点进行基因测序。结果显示:非洲水稻样本中有113个变异点,而亚洲水稻样本中有78个碱基对出现了变异;非洲水稻样本测序结果显示非洲水稻具有大聚集、小分散的特性,而亚洲水稻分布得较为广泛,由此可以证实非洲水稻与亚洲水稻在相互独立的进化演变过程中,第11号染色体Pik-2位点在LRR结构域形成了相似的单倍型。

Pik-2 locus is chromosome 11 of rice gene sequence.The variation and evolution of Pik-2 locus in rice genetic process were studied and analyzed.The natural evolution law of chromosome 11 in rice gene sequence and the inducing factors of malignant burst can be grasped,and the relevant control scheme can be worked out to improve the resistance gene of rice blast.The authors selected 400 rice samples from the African region and 400 samples from the Asian region,and sequenced the Pik-2 locus on chromosome 11 of all rice so as to find out that Africa There were 113 variations in rice samples and 78 base pairs in Asian rice samples.The results of sequencing of African rice samples showed that African rice had the characteristics of large aggregation and small dispersion,while Asian rice was more widely distributed.It can be concluded that the Pik-2 locus on chromosome 11 formed a similar haplotype in the LRR domain between African rice and Asian rice in the process of mutually independent evolution.