禁食及进餐前后对布氏鲳鲹缩胆囊素表达的影响

The Effect of Fasting Starvation Stress and Preprandial and Postprandial on the Expression of Cholecystokinin in Golden Pompano(Trachinotus blochii)

缩胆囊素(cholecystokinin, CCK)是生物体中抑制生物食欲的饱食信号因子,布氏鲳鲹属于摄食代谢十分旺盛的鱼类,揭示其CCK的表达规律能够进一步揭示鲳鲹的代谢机制。本研究开展了布氏鲳鲹禁食及餐前餐后养殖实验,并利用荧光定量PCR技术对布氏鲳鲹的缩胆囊素的表达模式进行了分析。在餐前餐后实验中,结果表明布氏鲳鲹下丘脑中的CCK的表达量在餐后0 h迅速上升达到峰值,随后在餐后1 h、3 h逐渐下降,并在+3 h下降至对照组水平。在禁食实验中,下丘脑中CCK的表达量在禁食阶段逐渐降低并在第5天下降至显著低于对照组,复投喂后在第7天又上升到对照组水平并保持至第11天。布氏鲳鲹CCK的表达对于饱食的信号反馈非常迅速,CCK的高表达与鲳鲹食欲控制相关。本研究结果可以为进一步研究布氏鲳鲹CCK基因的功能及调控提供理论参考。

Cholecystokinin(CCK)is a satiety signal factor that inhibits the biological appetite in organism.Golden pompano belongs to the fish that have very high ingestion metabolism,revealing the mechanism of action of CCK can provide support for studying the metabolic mechanism of golden pompano.In this study,the starvation and periprandial experiment were carried out,and we analyzed the expression model of CCK in golden pompano by using Real-time PCR.In preprandial and postprandial experiments,the result showed that the expression level of CCK in the hypothalamus of golden pompano increased gradually at 0 h and reached its peak.Subsequently,after a meal of 1 h and 3 h,the expression level of CCK decreased gradually and decreased to the control group level at 3 h after meal.During the fasting experiments,the expression level of CCK in the hypothalamus decreased gradually,and decreased to a significant lower expression level than control group on 5th day.After refeeding,the expression level of CCK in the hypothalamus of golden pompano increased and increased to the control group level on 7th day and held to 11th day.The expression of CCK in golden pompano is very rapid for the signal feedback of satiety,the high level expression of CCK of golden pompano is associated with appetite control.The results of this study can provide a theoretical reference for further research on the regulation and function of CCK gene in golden pompano.