七里香蔷薇叶绿体基因组测序及结构分析

Sequencing and Stuctural Analysis of Chloroplast Genome in Rosa banksias

七里香蔷薇是蔷薇属木香花的一个变种,在秦岭山区广泛分布,具有重要的观赏价值,同时也是蔷薇属花卉育种的重要种质资源。本研究首次以秦巴山区七里香蔷薇为实验材料,采用Illumina HiSeq4000测序系统对其叶绿体基因组DNA建库测序,建库类型为350 bp DNA小片段文库,测序深度为59×。分析其基因组结构,并且与已报道蔷薇科植物叶绿体基因组进行比较。七里香蔷薇叶绿体基因组序列全长156 544 bp,具有典型被子植物叶绿体基因组环状四分体结构。叶绿体基因组包括大单拷贝区85 678 bp、小单拷贝区18 782 bp和2个反向重复序列26 042 bp。GC含量为37.22%;检测到116个串联重复序列43个散在重复序列;此外,还检测到72个简单重复序列位点。对其功能基因比对分析,该植物的叶绿体基因组共注释得到129个基因,包括86蛋白编码基因、35个tRNA基因和8个rRNA基因。用MEGA6.0软件通过邻近法基于叶绿体全基因组序列对所有已知叶绿体基因组的蔷薇科22个物种进行聚类分析,其与七里香蔷薇和大花香水月季、缫丝花聚在一起。研究结果对于七里香蔷薇野生植物资源的育种及引种驯化具有参考依据。

Rosa banksias is a variety of Rosa banksiae in rose, which is widespread in the Qinling Mountains area.It has important ornamental value and is also an important germplasm resource for rose flower breeding. Rosa banksias in Qinba area was first used as experimental material and Illumina high-throughput sequencing technology was employed to sequence its chloroplast genome. The small fragments(350 bp) of DNA libraries were constructed according to the manufacturer’s manual for the Illumina Hi Seq 4000, and the sequencing depth was 59 fold. The genomic structure of the plant was analyzed and compared with the reported chloroplast genome of Rosaceae. The full length of chloroplast genome sequence of Rosa banksias is 156 544 bp, which has the cyclic tetrad structure of chloroplast genome of typical angiosperms, including large and small single copy regions(LSC and SSC) of 85 678 bp and 18 782 bp respectively, and a pair of inverted repeats(IRA and IRB) of 26 042 bp. The content of GC was 37.22%, 116 tandem repeat sequences and 43 dispersed repeats were detected in the repeat sequence. Additionally, 72 short simple repeats(SSRs) loci of cp genome were detected. Comparative analyzed its functional genes, a total of 129 predicted genes, including 86 protein-coding genes, 35 tRNA genes and 8 rRNA genes, were commented in the chloroplast genome of the plant. The cluster analysis of 22 species of Rosaceae of all known chloroplast genome with MEGA6.0 software based on chloroplast whole genome sequence, Rosa banksias var. noroalis had a closer genetic relationship with Rosa-roxburghii and Rosa-odorata-var-gigantea. The research results provided referential basis for the breeding and introduction of this wild plant.