摘要
基于甲醛(FA)与肼易发生亲核加成反应,从而阻断肼与荧光母体间电子传递的光诱导电荷转移(PET)机理,设计合成了以香豆素为母体的甲醛荧光探针3-苯并噻唑-7-肼香豆素(Coun)。实验结果显示,Coun与FA的最佳反应时间为4 min,极大地提高了检测效率,检测限为5×10^-6μmol/L。该探针具有良好的细胞膜通透性,可应用于生物领域,检测细胞内的甲醛含量。
A coumarin-based formaldehyde(FA)fluorescent probe Coun was designed and synthesized.The mechanism of this fluorescent probes for FA is based on blocking electron transfer between hydrazine and the dye upon FA nucleophilic addition.The results show that the optimal reaction time of Coun and FA is 4 min,which greatly improved the detection efficiency with detection limit of 5×10^-6μmol/L.In addition,the probe has good cell membrane permeability,and can be used in the biological field to detect endogenous formaldehyde.
作者
高漫
何鑫
崔京南
刘涛
田镇豪
何深贵
GAO Man;HE Xin;CUI Jingnan;LIU Tao;TIAN Zhenhao;HE Shengui(State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian,Liaoning 116024,China)
出处
《应用化学》
CAS
CSCD
北大核心
2019年第9期1053-1060,共8页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(21572029)项目资助~~
关键词
荧光探针
甲醛
响应快
细胞成像
fluorescent probe
formaldehyde
fast response
cell imaging
