基于液质联用技术的白头翁汤治疗溃疡性结肠炎小鼠血清代谢组学分析

Serummetabonomics Analysis of Ulcerative Colitis Mice Treated by Baitouweng Decoction Based on UPLC/Q-TOF-MS

目的研究白头翁汤给予溃疡性结肠炎小鼠前后内源性代谢物的变化,寻找与治疗溃疡性结肠炎有关的代谢生物标志物,探讨白头翁汤对溃疡性结肠炎模型的调节作用及其可能机制。方法采用DSS复制小鼠溃疡性结肠炎模型,借助超高效液相色谱串联四级杆飞行时间质谱(UPLC-Q-TOF/MS)技术,对各组小鼠血清样本进行测定,采用主成分分析法(PCA)和正交偏最小二乘法判别分析(OPLS DA)来筛选差异性代谢物。结果经分析,对照组、模型组和白头翁汤治疗组血清样本能够得到很好的区分,共鉴定出6种潜在生物标志物,白头翁汤给药后溃结小鼠的内源性代谢物水平发生不同程度的回调。结论白头翁汤可以使DSS诱导的溃结小鼠的异常代谢有所恢复,其治疗作用可能与机体内6个代谢物及3条相关代谢通路的调节有关。

OBJECTIVE To investigate the changes of endogenous metabolites before and after treatment of ulcerative colitis in mice with Baitouweng Decoction,to find metabolic biomarkers related to the treatment of ulcerative colitis,and to explore the regulation and the possible mechanisms of Baitouweng Decoction on ulcerative colitis model.METHODS The mouse model of ulcerative colitis was constructed by DSS.The serum samples of each group were determined by ultra high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry(UPLC-Q-TOF/MS).Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to screen for differential metabolites.RESULTS The serum samples of the control group,the model group and the Baitouweng Decoction group could be well distinguished.A total of six potential biomarkers were identified,and the endogenous metabolites of the mice were varying degrees of callbacks after the administration of Baitouweng Decoction.CONCLUSION Baitouweng Decoction could restore the abnormal metabolism of DSS induced ulcerated mice,and its therapeutic effect may be related to the regulation of six metabolites and three related metabolic pathways.