单核苷酸多态性与慢性HBV感染结局相关性分析

Association of single nucleotide polymorphisms with the outcome of chronic HBV infection

目的:探讨分析血清白细胞介素(IL)-8水平及IL-8基因启动子区-251A/T、-738T/A、-845T/C单核苷酸多态性与慢性HBV感染结局的相关性。方法:选取2014年2月至2017年2月接受治疗的HBV感染者203例,其中慢性HBV感染者102例为试验组,急性自限HBV感染者101例作为对照组,收集并提取患者外周静脉血DNA,采用聚合酶链反应技术检测IL-8基因启动子区-251A/T、-738T/A、-845T/C基因分布及等位基因频率,并采用酶联免疫吸附试验检测两组患者血清中IL-8的水平。结果:IL-8 251A/T单核苷酸形态多样性有AA、AT、TT基因型,试验组和对照组患者3种基因型频率和等位基因频率对比差异有统计学意义(P<0.05),携带A等位基因相比T基因患慢性HBV感染风险更高;IL-8-738T/A和IL-8-845T/C单核苷酸多态性均只发现TT基因型;试验组血清中IL-8水平高于对照组,且其感染程度越高,血清IL-8水平越高;AA基因型的慢性HBV感染者血清IL-8水平更高。结论:IL-8基因启动子区-251A/T单核苷酸多态性与慢性HBV感染者发病存在相关性,但与疾病严重程度无明显关系。A等位基因容易诱发慢性HBV感染,血清中IL-8水平与病情程度有关。IL-8-738T/A和IL-8-845T/C单核苷酸多态性与慢性HBV感染者是否发病没有相关性。

Objective:To investigate the correlation between serum IL-8 level and IL-8 gene promoter region-251A/T,-738T/A,-845T/C single nucleotide polymorphisms and chronic HBV infection outcome.Methods:A total of 203 patients with HBV infection who were treated in our hospital from February 2014 to February 2017 were selected.Among them,102 patients with chronic HBV infection(46 cases with mild hepatitis B mild,33 cases were moderate and severe in 31 cases)were selected as experimental group,101 cases of acute self-limiting HBV infection were used as control group,peripheral venous blood was collected and extracted,and the promoter region of IL-8 gene was detected by polymerase chain reaction(RT-8)-251A/T,-738T/A and-845T/C Distribution and allele frequency,and the levels of IL-8 in serum were measured by enzyme-linked immunosorbent assay(ELISA).Results:IL-8251-A/T single nucleotide have AA,the diversity of ats,TT genotype,experimental group and control group in three kinds of genome frequency and allele frequency comparison difference was statistically significant(P=0.014,P=0.023,P=0.015),compared to carry A allele T genetic risk for chronic HBV infection is higher;IL-8-738t/A and IL-8-845t/C single nucleotide polymorphism were found only in TT genotype;In the serum of chronic HBV infection group,IL-8 was higher than the control group,and the higher the degree of infection,the higher the indexes of serum.The serum IL-8 index of chronic HBV infected with genotype AA was higher.Conclusion:IL-8 gene promoter region-251A/T single nucleotide polymorphism is associated with the incidence of chronic HBV infection,but there is no significant relationship between the severity of disease.A allele is easy to induce chronic HBV infection,serum IL-8 indicator status and the degree of disease.IL-8-738T/A and IL-8-845T/C single nucleotide polymorphisms were not associated with the incidence of chronic HBV infection.