自噬相关蛋白LC3Ⅱ在非小细胞肺癌中的表达及其与放射治疗敏感性的相关性

Expression of autophagy-associated protein LC3II in non-small cell lung cancer and its correlation with radiotherapy sensitivity

目的研究自噬相关蛋白LC3II在非小细胞肺癌(non-small cell lung cancer,NSCLC,包括肺腺癌及鳞癌)中的表达情况,探讨自噬表达对肺癌放射治疗敏感性的影响及其意义。方法使用免疫组织化学方法检测LC3II在243例经统一方案治疗的NSCLC及48例正常肺组织中的表达,分析LC3II的表达以及与临床病理参数的关系,并按放疗疗效将患者分为4组,探讨LC3II表达与放疗敏感性的关系。结果①腺癌组、鳞癌组LC3II阳性表达均低于正常肺组织,差异有统计学意义(P<0.05);②LC3II表达与肿瘤大小、有无坏死及TNM分期相关,而与年龄、性别及病理类型无关。③与所有患者LC3II表达阳性率(52.7%)相比,完全缓解组在治疗前LC3II表达的阳性率最低(40.9%),而放疗无效组LC3II表达的阳性率最高(71.9%)。结论自噬相关蛋白LC3II在肺腺癌、鳞癌中表达下调,且与肿瘤大小、有无坏死及TNM分期相关,可能与肺癌的发生发展有关;其高表达与肺腺癌、鳞癌的放疗抵抗相关,在一定程度上影响放疗敏感性。

Objective To study the expression of autophagy-related protein LC3II in non-small cell lung cancer(NSCLC,including lung adenocarcinoma and squamous cell carcinoma),and elucidate the influence and significance of autophagy expression on lung cancer radiotherapy curative effect.Methods Immunohistochemical method was used to detect the expression of LC3-II in 243 cases of non-small cell lung cancer based on retrospective study and 48 cases of normal lung tissue,and analyze the the relationship with expression of LC3II and clinicopathological factors.Dividing the patients into four groups according to the curative effect,to explore the relationship between the expression of LC3II and radiotherapy sensitivity.Results①The positive expression of LC3II in the adenocarcinoma group and the squamous cell carcinoma group was lower than that in normal lung tissue.②High LC3II expression was found to be significantly correlated with tumor size,necrosis and TNM grade(P<0.05),but not with age,sex and pathological type.③Compared with all patients with positive expression of LC3II(52.7%),the positive rate of LC3II expression in CR group was the lowest(40.9%),whereas PD group was the highest(71.9%).Conclusion Autophagy-related protein LC3II was down-regulated in lung adenocarcinoma and squamous cell carcinoma,and associated with large size,necrosis and TNM grade,which may be related with the development of lung cancer.The high expression of autophagy is closely related to lung adenocarcinoma,squamous cell carcinoma and the radiation resistance,which would affect the efficacy of radiotherapy.