阿魏酸-米渣蛋白复合膜的结构及性能

Structural and Functional Properties of Ferulic Acid-Rice Residue Protein Composite Film

通过测定膜的机械性能、水蒸气透过率、水溶性研究了阿魏酸对米渣蛋白膜性能的影响,通过傅里叶红外光谱、差示扫描量热法、X-射线衍射、扫描电子显微镜表征了膜的结构特征。结果表明,当阿魏酸添加量为0.1%(W/V)时,与对照组相比,膜的抗拉强度提高20.33%,水蒸气透过率降低20.58%,水溶性降低23.88%。傅利叶红外光谱(FTIR)显示,添加阿魏酸后酰胺带吸收峰发生红移,β-折叠转变为易变的随机螺旋分子结构,蛋白质的二级结构发生改变;差示扫描量热法(DSC)分析表明阿魏酸增强了分子间的相互作用,提高了膜的玻璃化转变温度,使膜具有更好的热稳定性;X-射线衍射(XRD)分析表明阿魏酸与蛋白质分子间发生共价交联,膜的结晶度提高;扫描电镜(SEM)结果显示复配阿魏酸后的米渣蛋白膜的表面结构更均匀平整。因此,阿魏酸的添加使米渣蛋白膜具有更优的性能和更稳定的空间结构。

Effects of ferulic acid on the performance of rice residue protein films were studied by measuring the mechanical properties of the films,water vapor transmission rate and water solubility.The structural characteristics of the film were characterized by Fourier transform infrared spectroscopy,differential scanning calorimetry,X-ray diffraction,and scanning electron microscopy.Results showed that,with the content of 0.1%(W/V)ferulic acid,the tensile strength,the water vapor transmission rate and the water solubility of the composite RRP film were increased by 20.33%and decreased by 20.58%and 23.88%,respectively.Results of Fourier transform infrared spectroscopy(FTIR)showed that the amide band absorption peak was red-shifted,β-sheet was transformed into a flexible random helical molecular structure,and the secondary structure of the protein was changed by ferulic acid crosslinking.Differential scanning calorimetry(DSC)analysis showed that the ferulic acid enhanced the intermolecular interaction,it increased the glass transition temperature of the film,and the film had better thermal stability.X-ray diffraction(XRD)analysis showed that ferulic acid and protein molecules had covalent cross-linked,and the film crystallinity was increased.Scanning electron microscope(SEM)results displayed more uniform and smooth microstructure of the film.The addition of ferulic acid made RRP film indicated better performance and more stable spatial structure.