纯牛乳中硫氰酸钠高通量快速检测方法的建立

Development of A High Throughput Rapid Detection Method for Sodium Thiocyanate in Pure Milk

本研究基于硫氰酸根与三价铁离子反应生成血红色络合物的原理,以微孔板作为显色载体,通过显色剂筛选、显色条件优化、性能评定等步骤建立了一种准确、快速、高通量的纯牛乳中硫氰酸钠含量的检测方法。方法的工作条件为选取1.5 mol/L的高氯酸铁溶液(用2.0 mol/L高氯酸溶液配制)作为最佳显色剂,室温25℃下反应5 min,用酶标仪读取465 nm波长下的吸收值。结果显示:本方法检出限为0.27 mg/kg,定量限为0.90 mg/kg,线性范围为0.90~80.00 mg/kg,加标回收率范围在83.9%~98.6%之间,重复测试相对标准偏差均小于10.0%。本方法具有准确、通量高、试剂用量小、环保、快速等优点,能够满足纯牛乳中硫氰酸钠的检测,适合对大批量的纯牛乳中硫氰酸钠残留进行快速筛查。

Based on the principle of reaction of thiocyanate and ferric iron ions to form blood red complex,a high throughput rapid detection method for sodium thiocyanate in pure milk was developed by using microporus plate as color carrier through the steps of screening of color reagent,optimization of color condition and performance evaluation.Microporous plate was chosen for color developing carrier.The working conditions of this method were as follows:1.5 mol/L iron perchlorate solution(dissolved with 2.0 mol/L perchloric acid solution)served as chromogenic agent,reacting 5 min at 25℃,absorption values at 465 nm were recorded.The results showed that the limit of detection of this method was 0.27 mg/kg,the limit of quantification was 0.90 mg/kg,the linear range was 0.90~80.00 mg/kg,the addition recovery range was 83.9%~98.6%,and the relative standard deviation(RSD)of repeated tests was less than 10.0%.This method has the advantages of high accuracy and throughput,small amount of reagent,environment friendly,rapid,etc.,and is suitable for rapid screening of sodium thiocyanate residue for large quantities of pure milk samples.