暹罗鳄多肽粉对小鼠机体免疫力及抗疲劳效应的增强作用

Effects of peptide powder fromCrocodylus siamensis on enhancing immunity of organism and anti-fatigue effect in mice

为研究暹罗鳄(Crocodylus siamensis)多肽粉对小鼠免疫力及抗疲劳效应的影响,对小鼠的免疫力及相关血清指标、运动能力及相关酶活力指标进行了测定.实验结果显示:暹罗鳄多肽粉可以促进小鼠胸腺和脾脏的生长,增强机体免疫力.相对于对照组小鼠,暹罗鳄多肽粉组小鼠血清中的白蛋白、总蛋白、血钙含量升高(p<0.05),谷丙转氨酶活力和血糖浓度下降(p<0.05).小鼠爬杆及负重游泳实验发现服用了暹罗鳄多肽粉的小鼠垂直爬杆时间和负重游泳时间均长于对照组(p<0.05).进一步通过建立小鼠力竭游泳模型研究了暹罗鳄多肽粉的抗疲劳功效,结果显示与对照组相比,暹罗鳄多肽粉组小鼠血液中尿素氮和乳酸的含量以及乳酸脱氢酶活力均降低(p<0.05),而超氧化物歧化酶活力升高(p<0.05).综上结果表明暹罗鳄多肽粉可能有保肝降糖、增强机体免疫力及抗疲劳效应的作用.

In order to study the immunity and anti-fatigue effects of the peptide powder from Crocodylus siamensis on mice,the immunity and related serum indexes,and the exercise ability and related enzyme activities of mice were studied using the mouse model.The results showed that the growth of thymus and spleen of mice was promoted with the C.siamensis peptide powder and the immunity of organism was improved.In the C.siamensis peptide powder groups,compared with the control group,the levels of serum albumin,total protein,and calcium in mice increased(p<0.05),while the activitiy of alanine transaminase(ALT)and the concentration of blood glucose decreased(p<0.05).In addition,the results of climbing rope experiment and load swimming experiment showed that the time for vertical climbing rope and load swimming of mice in the C.siamensis peptide powder groups,were significantly longer than those in the control group(p<0.05).Then the anti-fatigue effect of the C.siamensis was analyzed by establishing exhaustive swimming mouse model,and results showed that the contents of urea nitrogen(BUN)and lactic acid(LA)in the blood reduced(p<0.05),the lactic dehydrogenase(LDH)activity decreased and the superoxide dismutase(SOD)activity increased(p<0.05).These results suggest that the peptide powder from C.siamensis might have the function of protecting liver,reducing blood glucose,improving the immunity of organism and anti-fatigue effect.