微生物多样性分析技术应用于食用菌发酵培养料分析的进展

Advances in Application of Microbial Diversity Analysis Techniques on Analyzing Edible Fungi Fermented Substrates

食用菌培养料的腐熟是由多种微生物共同作用而实现的,在这个过程中营养成分降解程度的差异,在一定程度上会影响后期的生产过程及产量。物种多样性差异反映了生态群落的功能和动态变化,因此分析发酵培养料制备过程中微生物多样性的变化对生产优质的培养料,使食用菌获得高产量尤为重要。本文梳理了食用菌发酵培养料微生物多样性分析的研究方法,简述了各种方法的原理并综述了其在相关研究中的应用,最后总结了不同方法的优缺点,以期为未来选用适宜的方法进行研究及开发复合菌制剂提供参考。

Edible fungi culture material is prepared by decomposing agricultural waste resources with microbes.During the process,the degree of nutrient degradation affects the subsequent production and yield at a certain level.The difference in nutrient degradation is a result of the microbial community in the culture material,and therefore it is particularly important to monitor the microbial species in the fermented substrates during the production process so as to provide references for preparing high quality culture substrates.In this review,various research approaches for microbial diversity analysis in edible fungi fermented substrates were reviewed for their principles and applications.The advantage and disadvantages of these techniques were also compared in order to provide reference for microbe diversity analysis method selection and high quality compound microbial formula development.