不同麻醉药对树胶脂毒素处理后的不同种属小鼠存活率的影响

Effects of different anesthetics on the survival rate of different species of resiniferatoxin-treated mice

目的观察两种常用麻醉剂水合氯醛和戊巴比妥钠在树胶脂毒素(resiniferatoxin,RTX)处理后对C57BL/6小鼠以及Kunming小鼠存活率的影响。方法C57BL/6小鼠24只,体质量24~28 g;Kunming小鼠55只,体质量28~35 g,采用随机数字表法分为4组:C57BL/6小鼠溶剂对照组(Ⅰ组,12只)、C57BL/6小鼠RTX组(Ⅱ组,12只)、Kunming小鼠溶剂对照组(Ⅲ组,10只)、Kunming小鼠RTX组(Ⅳ组,45只),4组小鼠连续3 d皮下注射RTX,2次/d,第1、2和3天的单次给药剂量分别为30μg/kg、70μg/kg和100μg/kg,每次注射RTX都提前给予防止呼吸衰竭的保护性混合物,每天观察小鼠死亡情况。2周后,Ⅰ组、Ⅱ组小鼠使用2%戊巴比妥钠(100 mg/kg,0.1 mL/20 g)麻醉,Ⅲ组、Ⅳ组使用10%水合氯醛(500 mg/kg,0.1 mL/20 g)麻醉,观察小鼠存活情况。结果在给予RTX或溶剂对照后,与Ⅰ组、Ⅳ组比较,Ⅱ组病死率显著增高(P<0.05);在给予不同麻醉药物麻醉后,与Ⅱ组、Ⅲ组比较,Ⅳ组病死率显著增高(P<0.01)。结论RTX对C57BL/6小鼠致死率较高,而水合氯醛会导致RTX处理后Kunming小鼠的死亡。

Objective To investigate the effect of chloral hydrate and pentobarbital sodium on the survival rate of C57BL/6 mice and Kunming mouse after resiniferatoxin(RTX)treatment.Methods Twenty four C57BL/6 mice weighing 24 28g;Fifty five Kunming mice weighing 28 35g were randomly divided into 4 groups:C57BL/6 mice solvent control group(groupⅠ,n=12),C57BL/6 mice RTX group(groupⅡ,n=12),Kunming mice solvent control group(groupⅢ,n=10),Kunming mice RTX group(groupⅣ,n=45).All animals were treated by subcutaneous injection of RTX for 3 consecutive days,twice daily,the single doses of 30,70 and 100μg/kg were given on day 1,2 and 3,respectively.Before RTX injection,mice were intraperitoneally injected(ip)a protective cocktail against respiratory failure in advance and the mortality of the mice was observed every day.Two weeks later,groupⅠand groupⅡmice were anesthetized with 2%pentobarbital sodium(100 mg/kg,0.1 mL/20 g,ip),groupⅢandⅣwere treated with 10%chloral hydrate(500 mg/kg,0.1 mL/20 g,ip)and the survival of the mice was observed.Results After subcutaneous injection of RTX,the mortality rate of groupⅡwas significantly increased(P<0.05),compared with groupⅠand groupⅣ.After anesthesia with different anesthetic drugs,the mortality rate of groupⅣwas significantly increased(P<0.01),compared with groupⅡand groupⅢ.Conclusion The lethal rate of RTX on C57BL/6 mice is relatively high,and chloral hydrate can cause the death of Kunming mice after RTX treatment.