RNU12 siRNA转染对EC9706细胞生物学特性的影响

Effects of RNU12 siRNA transfection on biological characteristics of EC9706 cells

目的:研究长链非编码RNA(LncRNA)RNU12 siRNA转染对食管癌EC9706细胞生物学特性的影响。方法:采用qRT-PCR检测EC9706细胞和人食管上皮细胞HEEC中RNU12的表达水平。将RNU12 siRNA转染EC9706细胞,同时设阴性对照组(转染随机序列)和空白对照组(未转染),采用qRT-PCR检测RNU12的表达,采用MTT法检测细胞增殖,Annexin V-FITC和PI双染法检测细胞凋亡,Transwell实验检测细胞侵袭和迁移能力。结果:EC9706细胞中RNU12的表达水平高于HEEC细胞(P<0.05)。与空白对照组和阴性对照组比较,RNU12 siRNA组细胞中RNU12的表达水平降低,细胞增殖能力受抑,凋亡率升高,侵袭和迁移细胞数减少(P<0.05)。结论:RNU12在EC9706细胞中高表达,干扰RNU12的表达能够抑制EC9706细胞增殖,促进细胞凋亡,阻碍细胞侵袭和迁移。

Aim:To investigate the effects of long non-coding RNA RNU12 on the biological characteristics of esophageal cancer EC9706 cells.Methods:The expression level of RNU12 in EC9706 cells and human esophageal epithelial cells HEEC was detected by qRT-PCR.RNU12 siRNA was transfected into EC9706 cells,and a negative control group(transfected random sequence)and a blank control group(not transfected)were set.The expression of RNU12 was detected by qRT-PCR.Cell proliferation was detected by MTT assay,apoptosis was detected by Annexin V-FITC and PI double staining,and Transwell assay was used to detect cell invasion and migration.Results:The expression level of RNU12 in EC9706 cells was higher than that in HEEC cells(P<0.05).Compared with the blank control group and the negative control group,the expression level of RNU12 was decreased in the RNU12 siRNA group,the cell proliferation ability was inhibited,the apoptosis rate was increased,and the number of invasion and migration cells was decreased(P<0.05).Conclusion:RNU12 is highly expressed in EC9706 cells.Interfering the expression of RNU12 could inhibit the proliferation of EC9706 cells,promote cell apoptosis,and hinder cell invasion and migration.