miR-142-3p表达对卵巢癌SKOV3细胞生长和转移的影响

Effect of miR-142-3p Expression on Growth and Metastasis of SKOV3 Ovarian Cancer Cells

目的探讨miR-142-3p表达对卵巢癌SKOV3细胞生长和转移的影响。方法收集40例卵巢癌患者标本,同时收集20例相应的癌旁组织。利用荧光定量PCR方法检测各组织中miR-142-3p表达水平。用脂质体作为载体将miR-142-3p模拟物转染至SKOV3细胞,应用实时PCR和Western blotting方法检测正常与与转染的SKOV3细胞HMGA2 mRNA及蛋白表达情况。结果miR-142-3p在卵巢癌组织中表达水平明显高于癌旁组织(P<0.01)。过表达miR-142-3p能够抑制HMGA2 mRNA和蛋白表达(P<0.05)。过表达miR-142-3p显著抑制SKOV3细胞生长和转移。结论miR-142-3p表达增加能够抑制SKOV3细胞中HMGA2 mRNA和蛋白表达,并抑制SKOV3细胞的生长和转移。

Objective To study the effects of miR-142-3p expression on the growth and metastasis of SKOV3 ovarian cancer cells.Methods Forty specimens from ovarian cancer patients were collected,with 20 corresponding adjacent tissue samples collected simul-taneously.The expression level of miR-142-3p in various tissues was determined by real-time quantitative PCR.miR-142-3p mimics were transfected into SKOV3 cells using a liposome vector.Real-time PCR and Western blotting were used to detect the expression of miR-142-3p in normal SKOV3 and transfected HMGA2 cells.Results The level of expression of miR-142-3p in ovarian cancer tissues was significantly higher than that of the adjacent tissues(P<0.01).Overexpression of miR-142-3p could inhibit the expression of HMGA2 mRNA and protein(P<0.05);additionally,this overexpression significantly inhibited the growth and metastasis of SKOV3 cells.Con-clusion Overexpression of miR-142-3p can inhibit the expression of both HMGA2 mRNA and protein,as well as inhibit the growth and metastasis of SKOV3 cells.