UPLC测定纤毛婆婆纳主要环烯醚萜苷成分的含量

Study on the content determination of main iridoid glycosides in Veronica ciliata Fisch by UPLC

建立超高效液相色谱法(UPLC)同时测定纤毛婆婆纳中梓醇、梓苷、胡黄连苷Ⅱ和6-O-藜芦酰梓苷4种主要环烯醚萜苷类成分含量.采用Waters ACOUITY T3色谱柱(100 mm×2.1 mm,1.8 μm);柱温为40 ℃;流动相为乙腈-水(0.1%甲酸+2.5 mmol甲酸铵);流速为0.4 mL/min;检测波长为260 nm.结果表明梓醇、梓苷、胡黄连苷Ⅱ和6-O-藜芦酰梓苷4种主要环烯醚萜苷类成分含量.结果表明梓醇、梓苷、胡黄连苷Ⅱ和6-O-藜芦酰梓苷分别在1.562~50 μg/mL( R 2 =0.9987)、1.562~50 μg/mL( R 2 =0.9989)、4.689~143 μg/mL( R 2 =0.9996)、1.562~50 μg/mL( R 2 =0.9998)范围内呈现良好的线性关系,平均回收率分别为100.21%(RSD=1.3%)、99.43%(RSD=1.8%)、100.6%(RSD=2.5%)、102.7%(RSD=0.65%).该方法简便、灵敏、经济、可靠,可用于纤毛婆婆纳的质量控制和评价.

A UPLC method has been developed in the current investigation for simultaneous determination of four main iridoid glycosides,which were verproside,catalposide,amphicoside and 6-O-veratroylcatalposide in Veronica ciliata Fisch.The separation was performed at 40℃on Waters ACOUITY T3 column(100 mm×2.1 mm,1.8μm);the mobile phase was acetonitrile-water(0.1%formic acid+2.5 mmol ammoniumformate);the flow rate was 0.4 mL/min and the detection wavelength was 260 nm.The results showed that the calibration curves were the good linear relationships in the range of 1.562~50μg/mL(R 2=0.9987)for verproside,1.562~50μg/mL(R 2=0.9989)for catalposide,4.689~143μg/mL(R 2=0.9996)for amphicoside,1.562~50μg/mL(R 2=0.9998)for 6-O-veratroylcatalposide,the average recovery rate were 100.21%(RSD=1.3%),99.43%(RSD=1.8%),100.6%(RSD=2.5%),102.7%(RSD=0.65%),respectively.The method is simple,sensitive,economical and reliable,and can be used for quality control and evaluation of Veronica ciliata Fisch.