摘要
试验旨在研究富维生素E(VE)抗虫抗除草剂转基因玉米(富VE转基因玉米)对肉鸡生长性能、生化指标及抗氧化功能的影响,并与非转基因玉米添加外源DL-α-生育酚乙酸酯作用效果比较,对富VE转基因玉米营养有效性进行评价。选用360只1日龄罗斯308肉鸡公雏,随机分为3组,每组6个重复,每个重复20只,试验期42 d。普通玉米负对照组(负对照组,NC)日粮使用非转基因玉米配制不添加外源VE,1~21和22~42 d日粮VE水平分别为4.38和4.63 IU/kg;富VE转基因玉米组(富VE组,VER)使用富VE转基因玉米配制,1~21和22~42 d日粮VE水平分别为14.11和14.91 IU/kg;普通玉米正对照组(正对照组,PC)使用非转基因玉米配制并添加DL-α-生育酚乙酸酯添加剂,使其VE水平与富VE组一致。结果表明:①除1~21 d富VE组平均日采食量(ADFI)显著低于负对照组外(P<0.05),其他生长性能各指标在各组间均无显著性差异(P>0.05)。②21 d时,富VE组血清谷丙转氨酶(ALT)与正对照组相比无显著差异(P>0.05),与负对照组相比显著降低(P<0.05);肝脏甘油三酯(TG)含量低于负对照组但无显著性差异(P>0.05),且两组均显著高于正对照组(P<0.05);42 d时,富VE组肝脏TG含量与正对照组相比无显著性差异(P>0.05),且两组均显著低于负对照组(P<0.05)。③与正对照组相比,富VE组21 d血清丙二醛(MDA)、过氧化氢(H 2O 2)、还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)以及42 d血清MDA、H 2O 2、GSH、SOD、CAT和T-AOC均无显著性差异(P>0.05);与负对照组相比,富VE组21 d和42 d血清MDA和H 2O 2均显著降低(P<0.05),21 d血清GSH、GSSG、谷胱甘肽过氧化物酶(GSH-Px)、SOD、CAT和T-AOC及42 d血清GSSG、GSH-Px、CAT和T-AOC均显著升高(P<0.05)。以上结果表明,同等VE水平条件下,富VE转基因玉米与非转基因玉米添加外源DL-α-生育酚乙酸酯添加剂对试验肉鸡生长性能、生化指标和抗氧化功能具有相似作用效果。
This study was conducted to investigate the effects of VE-riched transgenic maize on growth performance,biochemical parameters and antioxidant capacity of broilers,and evaluate the nutritional effectiveness of VE-riched transgenic maize compared with the non-genetically modified maize(non-GM maize)added exogenous DL-α-tocopherol acetate additive.360 1-day-old Ross 308 male broilers were randomly divided into 3 groups with 6 replicates and 20 chickens in per replicate.The study period was 42 d.The diet for ordinary maize negative control group(negative control group,NC)was prepared with non-GM maize without exogenous VE and the VE level of diets during 1 to 21 d and 22 to 42 d were 4.38 and 4.63 IU/kg,respectively;Diet for VE-riched transgenic maize group(VE-riched group,VER)was prepared with VE-riched transgenic maize and the VE level of diets during 1 to 21 d and 22 to 42 d were 14.11 and 14.91 IU/kg,respectively;Diet for ordinary maize positive control group(positive control group,PC)was prepared with non-GM maize added DL-α-tocopherol acetate additive to make VE level consistent with VE-riched group.The results showed as follows:①Except for average daily feed intake(ADFI)of 1 to 21 d in VE-riched group which was significantly lower than that in negative control group(P<0.05),there was no significant difference in other growth performance parameters among the three groups during the experiment(P>0.05).②On 21 d,the serum ALT of VE-riched group was not significantly different from positive control group(P>0.05),but significantly lower than that of negative control group(P<0.05),and there was no significant difference in liver TG content between VE-riched group and negative control group(P>0.05),and the two groups were significantly higher than that of positive control group(P<0.05);On 42 d,there was no significant difference in the liver TG content between VE-riched group and positive control group(P>0.05),and the two groups were significantly lower than that of negative control group(P<0.05);③Compared with positive control group,serum MDA,H 2O 2,GSH,GSSG,SOD,CAT and T-AOC of 21 d and serum MDA,H 2O 2,GSH,SOD,CAT and T-AOC of 42 d in VE-riched group had no significant difference(P>0.05);Compared with negative control group,serum MDA and H 2O 2 of 21 d and 42 d significantly decreased in VE-riched group(P<0.05),meanwhile,GSH,GSSG,GSH-Px,SOD,CAT and T-AOC of 21 d and GSSG,GSH-Px,CAT and T-AOC of 42 d increased significantly in the VE-riched group(P<0.05).The above results demonstrated that VE-riched transgenic maize and non-GM maize added with exogenous DL-α-tocopherol acetate additive had similar effects on growth performance,biochemical parameters and antioxidant capacity of the test broilers under the same VE level.
作者
詹腾飞
韩云胜
汤超华
赵青余
张军民
ZHAN Tengfei;HAN Yunsheng;TANG Chaohua;ZHAO Qingyu;ZHANG Junmin(State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China,Ministry of Agriculture and Rural Affairs,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《中国畜牧兽医》
CAS
北大核心
2019年第9期2608-2617,共10页
China Animal Husbandry & Veterinary Medicine
基金
转基因生物新品种培育科技重大专项(2016ZX08003002)
关键词
富维生素E转基因玉米
肉鸡
生长性能
生化指标
抗氧化功能
VE-riched transgenic maize
broiler
growth performance
biochemical parameters
antioxidant capacity