富维生素E转基因玉米对肉鸡生长性能、生化指标及抗氧化功能的影响

Effects of Vitamin E-riched Transgenic Maize on Growth Performance, Biochemical Parameters and Antioxidant Capacity of Broilers

试验旨在研究富维生素E(VE)抗虫抗除草剂转基因玉米(富VE转基因玉米)对肉鸡生长性能、生化指标及抗氧化功能的影响,并与非转基因玉米添加外源DL-α-生育酚乙酸酯作用效果比较,对富VE转基因玉米营养有效性进行评价。选用360只1日龄罗斯308肉鸡公雏,随机分为3组,每组6个重复,每个重复20只,试验期42 d。普通玉米负对照组(负对照组,NC)日粮使用非转基因玉米配制不添加外源VE,1~21和22~42 d日粮VE水平分别为4.38和4.63 IU/kg;富VE转基因玉米组(富VE组,VER)使用富VE转基因玉米配制,1~21和22~42 d日粮VE水平分别为14.11和14.91 IU/kg;普通玉米正对照组(正对照组,PC)使用非转基因玉米配制并添加DL-α-生育酚乙酸酯添加剂,使其VE水平与富VE组一致。结果表明:①除1~21 d富VE组平均日采食量(ADFI)显著低于负对照组外(P<0.05),其他生长性能各指标在各组间均无显著性差异(P>0.05)。②21 d时,富VE组血清谷丙转氨酶(ALT)与正对照组相比无显著差异(P>0.05),与负对照组相比显著降低(P<0.05);肝脏甘油三酯(TG)含量低于负对照组但无显著性差异(P>0.05),且两组均显著高于正对照组(P<0.05);42 d时,富VE组肝脏TG含量与正对照组相比无显著性差异(P>0.05),且两组均显著低于负对照组(P<0.05)。③与正对照组相比,富VE组21 d血清丙二醛(MDA)、过氧化氢(H 2O 2)、还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)以及42 d血清MDA、H 2O 2、GSH、SOD、CAT和T-AOC均无显著性差异(P>0.05);与负对照组相比,富VE组21 d和42 d血清MDA和H 2O 2均显著降低(P<0.05),21 d血清GSH、GSSG、谷胱甘肽过氧化物酶(GSH-Px)、SOD、CAT和T-AOC及42 d血清GSSG、GSH-Px、CAT和T-AOC均显著升高(P<0.05)。以上结果表明,同等VE水平条件下,富VE转基因玉米与非转基因玉米添加外源DL-α-生育酚乙酸酯添加剂对试验肉鸡生长性能、生化指标和抗氧化功能具有相似作用效果。

This study was conducted to investigate the effects of VE-riched transgenic maize on growth performance,biochemical parameters and antioxidant capacity of broilers,and evaluate the nutritional effectiveness of VE-riched transgenic maize compared with the non-genetically modified maize(non-GM maize)added exogenous DL-α-tocopherol acetate additive.360 1-day-old Ross 308 male broilers were randomly divided into 3 groups with 6 replicates and 20 chickens in per replicate.The study period was 42 d.The diet for ordinary maize negative control group(negative control group,NC)was prepared with non-GM maize without exogenous VE and the VE level of diets during 1 to 21 d and 22 to 42 d were 4.38 and 4.63 IU/kg,respectively;Diet for VE-riched transgenic maize group(VE-riched group,VER)was prepared with VE-riched transgenic maize and the VE level of diets during 1 to 21 d and 22 to 42 d were 14.11 and 14.91 IU/kg,respectively;Diet for ordinary maize positive control group(positive control group,PC)was prepared with non-GM maize added DL-α-tocopherol acetate additive to make VE level consistent with VE-riched group.The results showed as follows:①Except for average daily feed intake(ADFI)of 1 to 21 d in VE-riched group which was significantly lower than that in negative control group(P<0.05),there was no significant difference in other growth performance parameters among the three groups during the experiment(P>0.05).②On 21 d,the serum ALT of VE-riched group was not significantly different from positive control group(P>0.05),but significantly lower than that of negative control group(P<0.05),and there was no significant difference in liver TG content between VE-riched group and negative control group(P>0.05),and the two groups were significantly higher than that of positive control group(P<0.05);On 42 d,there was no significant difference in the liver TG content between VE-riched group and positive control group(P>0.05),and the two groups were significantly lower than that of negative control group(P<0.05);③Compared with positive control group,serum MDA,H 2O 2,GSH,GSSG,SOD,CAT and T-AOC of 21 d and serum MDA,H 2O 2,GSH,SOD,CAT and T-AOC of 42 d in VE-riched group had no significant difference(P>0.05);Compared with negative control group,serum MDA and H 2O 2 of 21 d and 42 d significantly decreased in VE-riched group(P<0.05),meanwhile,GSH,GSSG,GSH-Px,SOD,CAT and T-AOC of 21 d and GSSG,GSH-Px,CAT and T-AOC of 42 d increased significantly in the VE-riched group(P<0.05).The above results demonstrated that VE-riched transgenic maize and non-GM maize added with exogenous DL-α-tocopherol acetate additive had similar effects on growth performance,biochemical parameters and antioxidant capacity of the test broilers under the same VE level.