浙江地区儿童感染人巨细胞病毒gH基因分型研究

Study on the genotyping of gH gene among children with human cytomegalovirus infection in Zhejiang

目的了解浙江地区儿童感染人巨细胞病毒(HCMV)糖蛋白H(gH)基因不同型别的分布特征。方法收集2015年1月—2017年12月浙江大学医学院附属儿童医院HCMV DNA检测阳性患儿尿液样本、咽拭子样本或患儿母亲乳汁样本,采用荧光定量聚合酶链反应(PCR)对gH基因进行分型检测。按照年龄、样本类型、疾病类型对HCMV阳性患儿进行分组,比较各组gH基因类型分布。结果1 232例HCMV DNA阳性样本中,gH1型766例(62.2%),gH2型373例(30.3%),gH1和gH2混合型93例(7.5%)。不同年龄、样本类型及疾病类型患儿gH基因类型分布趋势一致,无统计学差异(P>0.05)。结论浙江地区儿童感染HCMV以gH1型为主,不同年龄、样本类型及疾病类型患儿HCMV gH基因类型无差异。

Objective To investigate the distribution characteristics of human cytomegalovirus(HCMV)glycoprotein H(gH)genotyping among children in Zhejiang.Methods Urine,throat swab or mothers'milk sample was collected from children with positive HCMV DNA of Children's Hospital of Zhejiang University School of Medicine from January 2015 to December 2017.The genotyping of gH gene was performed using fluorescence quantitation polymerase chain reaction(PCR).Children with positive HCMV DNA were classified according to ages,sample types and diseases.The genotype distribution of gH gene was compared.Results The gH genotyping was performed in 1 232 positive HCMV samples.There were 766(62.2%)cases of gH1,373(30.3%)cases of gH2 and 93(7.5%)cases of gH1+gH2.The distribution trend of gH genotyping in positive HCMV children with different ages,different sample types and different diseases was consistent,without statistical significance(P>0.05).Conclusions HCMV infection is mainly caused by gH1 in Zhejiang,and the distribution of HCMV gH genotypes shows no statistical significance in different ages,different sample types and different diseases.