摘要
针对农业活动中广泛使用的杀螟丹、螺虫乙酯和常见重金属污染物铜、镉,以费氏弧菌为受试生物研究污染物并存状态下的联合毒性。通过研究在不同暴露时间下二元复合污染对费氏弧菌的急性毒性效应,计算半数效应浓度EC50,并采用混合毒性指数法评价联合毒性。研究表明,铜、镉、杀螟丹、螺虫乙酯单一暴露于费氏弧菌15 min的EC50分别为0.53、0.74、79.06 mg·L^-1和116.67 mg·L^-1。杀螟丹和重金属的混合体系对费氏弧菌的联合毒性主要表现为相加作用,当螺虫乙酯在混合体系中占比较低时,也表现为部分相加作用。杀螟丹和螺虫乙酯能够减缓金属离子进入细胞的速率,因此费氏弧菌急性毒性实验研究联合毒性时,暴露时间应延长至45 min以上。
In view of the widespread use of cartap,spirotetramat,and common heavy metal pollutants,such as copper and cadmium in agricultural activities,the joint toxicity of their complexes on Vibrio fischeri was studied.The EC50(median effective concentration)was calcu?lated with the acute toxic effects of binary complex contamination on Vibrio fischeri employing different exposure times.The Mixtures Toxicity Index method was employed to evaluate the joint toxicity.The acute toxicity experiments showed that the EC50 of copper,cadmium,cartap,and spirotetramat on Vibrio fischeri at 15 mins was 0.53,0.74,79.06 mg·L^-1,and 116.67 mg·L^-1,respectively.The joint toxicity of cartap and heavy metals on Vibrio fischeri was mainly additive.When the spirotetramat accounted for a low proportion in the binary mixtures,the joint toxicity was partially additive.The presence of cartap and spirotetramat may slow down the rate of metal ions entering the cell.Therefore,the study of exposure time for joint toxicity on Vibrio fischeri should be extended to 45 mins.
作者
殷鸿洋
赵远
郑义
保聪
黄新新
丁英杰
蔡强
YIN Hong-yang;ZHAO Yuan;ZHENG Yi;BAO Cong;HUANG Xin-xin;DING Ying-jie;CAI Qiang(College of Environment&Safety Engineering,Changzhou University,Changzhou 213164,China;Zhejiang Yangtze Delta Region In?stitute of Tsinghua University,Jiaxing 314006,China;Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;Wageningen University,Marine Animal Ecology Group,De Elst 1,Wageningen 6708WD,The Netherlands;Shanghai Entry-Exit In?spection and Quarantine Bureau,Technology Center Animal,Plant and Food Inspection and Quarantine,Shanghai 200135,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2019年第9期2080-2085,共6页
Journal of Agro-Environment Science
基金
国家重点研发项目(2017YFC1700800)
广东省省级科技计划项目(2017A020217009)
嘉兴市科技计划项目(2017AY33082)
关键词
重金属
农药
费氏弧菌
急性毒性
联合毒性评价
heavy metals
pesticide
Vibrio fischeri
acute toxicity
joint toxicity evaluation