饲料中添加壳聚糖对方斑东风螺稚螺生长性能和免疫相关酶活性的影响

Effects of chitosan on growth performance and immune-related enzymes activities of Babylonia areolata

研究旨在观察探究梯度添加壳聚糖(chitosan)对方斑东风螺(Babylonia areolata)稚螺生长性能和免疫相关酶活性的影响探讨。选用体重0.9 g左右的方斑东风螺稚螺480颗,随机分到12个养殖试验箱,每个箱中放入螺苗40颗,分别饲喂添加0、0.2%、0.6%、1%含量壳聚糖的饲料,日饲喂量约为螺总重的5%左右,饲养试验时间为90 d。结果表明,1%和0.6%壳聚糖添加组方斑东风螺稚螺的成活率分别达到了94.17%和92.5%,明显高于对照组,差异显著(P<0.05);各壳聚糖添加组与对照组相比在增重率、肥满度、肉壳比、脏体比都没有出现显著性差异(P>0.05);0.6%壳聚糖添加组方斑东风螺肝胰脏中的AKP、ACP活性明显高于对照组和1%添加组,差异显著(P<0.05);0.2%壳聚糖添加组的方斑东风螺肝胰脏中溶菌酶的活性明显高于对照组和0.6%添加组,差异显著(P<0.05)。因此,饲料中添加壳聚糖可以通过提高方斑东风螺稚螺肝脏中免疫相关酶的活性提高饲养成活率,并能够在一定程度上加快生长速度。

The purpose of this study was to investigate the effects of chitosan gradient on growth performance and immune-related enzymes activities ofBabylonia areolata. 480 B. areolata weighingabout 0.9 g were randomly divided into 12 cul-ture test boxes. 40 B. areolata were placed ineach box and fed with diets containing 0, 0.2%,0.6% and 1% chitosan respectively. The dailyfeeding amount was about 5% of the total B. are-olata and the feeding test time was 90 days. The results showed that the survival rates of B. areolata with 1% and 0.6% chitosan were 94.17% and92.5% respectively, which were significantly higher than those of the control group(P<0.05). Therewere no significant differences in weight gain, fatness, meat-to-chitosan ratio and viscera ratio be-tween the two groups(P>0.05). AKP and ACP activities in liver and pancreas of snails with 0.6% chi-tosan addition were significant(P<0.05). The activity of lysozyme in liver and pancreas of 0.2% chito-san group was significantly higher than that of control group and 0.6% chitosan group(P<0.05). Theresults indicated that the addition of chitosan in the feed could increase the survival rate of B. areolataby increasing the activity of immune-related enzymes in the liver, and to a certain extent acceleratethe growth rate.