基于PAC修饰的氧化石墨烯负载纳米Pt构建新型三维过氧化氢电化学传感器

Construction of a Novel 3D Hydrogen Peroxide Electrochemical Biosensor Based on PAC Modified GO/GCE Supported with Nano-Pt

为构建一种新的无酶电化学生物传感器并将其用于细胞中释放的H2O2的有效检测,将聚二烯丙基二甲基氯化铵(PAC)修饰的氧化石墨烯(GO)和纳米Pt通过物理吸附和电化学沉积H2PtCl6共同负载于玻碳电极上,成功制备了Pt/PAC-GO/GCE生物传感器。扫描电镜表明纳米Pt能很好地负载于PAC-GO表面。Pt/PAC-GO/GCE生物传感器对H2O2具有较宽的线性范围(0.04~3.6μmol/L及4.4~28.6μmol/L)及较高的电流响应(检出限为0.01μmol/L)。此外,该生物传感器具有较强的抗干扰能力,已成功用于活细胞H2O2的检测,有望用于医学诊断。

In order to construct a new non-enzymatic electrochemical biosensor and use it for the effective detection of the release of H2O2 from cells,the graphene oxide(GO)modified by polydiallyl dimethyl ammonium chloride(PAC)and nano-Pt were immobilized on glass carbon electrode(GCE)through the physical adsorption and electrodeposition of H2PtCl6 solution,respectively,and a Pt/PAC-GO/GCE electrochemical biosensor was successfully prepared.Through the characterization of scanning electron microscopy(SEM)and energy-dispersive X-ray spectroscopy(EDS),it was observed that the electrodeposited nano-Pt were densely covered and well distributed on the PAC-GO surface.Under the optimal experimental conditions,the Pt/PAC-GO/GCE biosensor exhibited a high peak current toward H2O2 and wide linear ranges for H2O2 in the range of 0.04-3.6μmol/L and 4.4-28.6μmol/L,with a detection limit of 0.01μmol/L.Moreover,the Pt/PAC-GO/GCE biosensor exhibited excellent anti-interference capability and reproducibility for the detection of H2O2,and was also successfully used in the detection of H2O2 in different living cell.All these results proved that the prepared biosensor has a potential application in clinical diagnostics.