主动脉瓣反流合并左心室增大患者外周血差异表达环状RNAs的筛选

Screening of differential expression of circRNA in peripheral blood of patients with aortic regurgitation and left ventricular enlargement

目的筛选主动脉瓣反流合并左心室增大患者外周血差异表达的环状RNA(circRNAs).方法选择4例主动脉瓣反流合并左心室增大患者(观察组)、4例主动脉瓣反流无左心室增大患者(对照组),均抽取空腹静脉血,采用高通量circRNA芯片技术检测二者外周血circRNAs,筛选二者外周血差异表达circRNAs,采用Target Scan、miRan-da软件预测分析与心肌重构相关miRNA-233有关联的circRNAs.结果观察组158个circRNA的荧光信号强度FC值均>2,且与对照组比较,P均<0.05;其中105个表达下调、53个表达上调,21个circRNAs的FC值>5.158个差异表达circRNAs中,hsa-circRNA-102898,hsa-circRNA-101661,hsa-circRNA-103740与miRNA-233存在结合位点.结论与主动脉瓣反流无左心室增大患者比较,主动脉瓣反流合并左心室增大患者外周血存在158个差异表达circR-NAshsa-circRNA-103245、hsa-circRNA-104014、hs-circRNA-102617、hsa-circRNA-103421等21个circRNAs表达差异明显.158个差异表达circRNAs可能与主动脉瓣反流合并左心室增大的发生、发展有关.hsa-circRNA-102898,hsa-cir-cRNA-101661,hsa-circRNA-103740可能通过与miRNA-233位点结合调控左心室增大.

Objective To screen out the differential expression circRNA in peripheral blood of patients with aortic regurgitation and left ventricular enlargement.Methods Four patients with aortic regurgitation complicated with left ventricular enlargement(observation group)and four patients with aortic valve regurgitation without left ventricular enlargement(control group)were selected.Their fasting venous blood was taken to detect the circRNAs by high throughput circRNA chip technique,and differentially expressed circRNAs between the two groups were screened out.Target Scan ant miRanda software were used to predict the circRNAs which were associated with miRNA-233 related to myocardial remodeling.Results Compared with the control group of fluorescence signal intensity,158 circRNAs were differentially expressed,with fold change values>2(P<0.05)including 21 circRNAs'fold change values>5,meanwhile,105 were down-regulated and 53 were up-regulated.There were binding sites between hsa-circRNA-102898,hsa-circRNA-101661,hsa-circRNA-103740 and miRNA-233 of 158 differentially expressed circRNAs.Conclusions Compared with patients with aortic regurgitation without left ventricular enlargement,there are 158 differentially expressed circRNAs in peripheral blood of patients with aortic regurgitation and left ventricular enlargement.Twenty-one circRNAs,such as hsa-circRNA-103245,hsacircRNA-104014,hs-circRNA-102617,and hsa-circRNA-103421 are significantly different.Totally 158 differential expressed circRNAs may be associated with the occurrence and development of left ventricular enlargement with aortic regurgitation.The hsa-circRNA-102898,hsa-circRNA-101661,and hsa-circRNA-103740 may regulate left ventricular enlargement by binding to miRNA-233 site.