阴道镜活检联合P16、OLFM4诊断宫颈上皮内瘤变的价值观察

The value of colposcopy biopsy combined with P16 and OLFM4 for diagnosing women with cervical intraepithelial neoplasia

目的:观察阴道镜活检联合P16、OLFM4诊断宫颈上皮内瘤变(CIN)的价值。方法选择2017年4月—2019年4月因宫颈病变于本院妇科门诊就诊,并行阴道镜活检的宫颈上皮内瘤变(CIN)患者240例,根据收集的病理标本诊断结果分为CINI(98例)、CINⅡ(82例)、CINⅢ(60例)。并选取60例因子宫良性病变切除的宫颈标本作为对照组。观察阴道镜活检结果,并采用免疫组化法检测P16、OLFM4的表达。结果:阴道镜活检诊断符合率CINI为91.7%、CINⅡ为90.2%,CINⅢ为96.7%;P16、OLFM4在对照组中阳性表达率低于CIN各组(P<0.05);P16、OLFM4在阳性表达率CINI最低,CINⅡ次之,CINⅢ最高(P<0.05)。结论:阴道镜活检联合P16、OLFM4检测可提高CIN的检出率,P16、OLFM4过表达与CIN的发生、发展及预后有关。

Objective:To observe the value of colposcopy biopsy combined with P16 and OLFM4 for diagnosing women with cervical intraepithelial neoplasia(CIN).Methods:From April 2017 to April 2019,240 patients with CIN who underwent colposcopy biopsy were selected into study group,which included 98 cases with CIN I,82 cases with CIN Ⅱ and 60 cases with CIN Ⅲ based on the pathological diagnostic results.Another 60 patients with cervical benign lesion were selected into control group.The results of colposcopy biopsy of all patients were observed.The expressions of P16 and OLFM4 were detected by immunohistochemistry.Results:The coincidence rates of CIN I,CIN Ⅱ and CIN Ⅲ diagnosis by colposcopy biopsy were 91.7%,90.2%,and 96.7%.The positive expression rates of P16 and OLFM4 of women in the control group were significant lower than those of women in the control group(P<0.05).The positive expression of P16 and OLFM4 was the lowest in the patients with CIN I and was the highest in the patients with CIN III(P<0.05).Conclusion:Colposcopy biopsy combined with the expression of P16 and OLFM4 can increase the detection rate of CIN.Excessive expressions of P16 and OLFM4 are closely related to the occurrence,development and prognosis of CIN.