期刊文献+

棉花FAD2-1基因的RNAi载体构建及高油酸新种质的获得 被引量:2

Construction of RNAi Vector of Cotton (Gossypium hirsutum L.) FAD2-1 Gene and Acquisition of New High Oleic Acid Germplasm
下载PDF
导出
摘要 【目的】在棉花纤维产量和品质不受影响的前提下,创造高油酸、低亚油酸棉花新种质。【方法】棉花FAD2-1基因是催化单不饱和脂肪酸的油酸形成多不饱和脂肪酸的亚油酸的关键基因,采用信息分析学方法分析其蛋白质性质、结构、功能;克隆GhFAD2-1的保守片段381 bp,构建RNA干涉载体,采用农杆菌介导的下胚轴侵染方法将干涉载体转入棉花。利用气相色谱-质谱联用仪(Gas chromatography-mass spectrometer,GC-MS)测定转基因T2~T4植株种子的脂肪酸成分及含量。对T4转基因株系进行拷贝数鉴定、目的基因表达量检测、农艺性状及纤维品质考察。【结果】成功构建GhFAD2-1的RNA干涉载体并转入棉花,转基因株系目的基因GhFAD2-1的表达量显著低于对照,具有高油酸、低亚油酸的表型并且能稳定遗传给后代;转基因株系可将棉花种子油酸提高224.1%,将亚油酸降低237.5%。与对照材料相比,转基因株系的农艺性状、纤维品质没有明显差异。【结论】验证了棉花GhFAD2-1基因的功能并且为培育高油酸棉花品种提供了帮助。 [Objective]Here,the aim was to create a new cotton(Gossypium hirsutum L.)germplasm with high oleic acid and low linoleic acid contents without affecting the fiber yield and quality.[Method]The fatty acid desaturation 2-1(FAD2-1)gene catalyzes the formation of polyunsaturated linoleic acid from monounsaturated fatty acid in cotton.The protein’s properties,structure and functions were analyzed using the information analysis method.A conserved 381-bp fragment of GhFAD2-1 was cloned to construct an RNA interference vector,which was transformed into cotton by Agrobacterium-mediated hypocotyl infection.The fatty acid compositions and contents of T2―T4 transgenic plants were determined using gas chromatography-mass spectrometry.The T4 transgenic lines were used to investigate the copy number,target gene expression,agronomic traits,and fiber quality.[Results]The RNA interference vector of GhFAD2-1 was successfully constructed and transformed into cotton.The target gene’s expression level was significantly lower in transgenic plants than in controls.The transgenic plants had high oleic acid and low linoleic acid contents,which could be stably inherited by the offspring.The oleic acid contents of transgenic cotton seeds increased by 224.1%,and the linoleic acid content decreased by 237.5%,compared with the control seeds.There were no significant differences in the agronomic traits and fiber quality of the transgenic lines compared with the control.[Conclusion]These results verified the function of GhFAD2-1 in cotton and provide a basis for breeding cotton varieties with high oleic acid contents.
作者 徐孝兰 许忠平 郭小平 Xu Xiaolan;Xu Zhongping;Guo Xiaoping(College of Plant Science and Technology,Huazhong Agricultural University/National Key Laboratory of Crop Genetic Improvement,Wuhan 430070,China)
出处 《棉花学报》 CSCD 北大核心 2019年第5期394-402,共9页 Cotton Science
基金 转基因生物新品种培育科技重大专项(2016ZX08005-005)
关键词 棉花 FAD2-1 脂肪酸 油酸 亚油酸 cotton FAD2-1 fatty acid oleic acid linoleic acid
  • 引文网络
  • 相关文献

参考文献1

二级参考文献10

共引文献7

同被引文献20

引证文献2

二级引证文献2

相关主题

;
使用帮助 返回顶部