染色质重塑因子在植物发育过程的功能

Functions of ATP-dependent Chromatin Remodeling Factors in Plant Development

染色质重塑复合体(chromatin remodeling complexes)通过具有ATPase活性的亚基水解ATP释放能量,通过改变核小体“构象”(包括核小体重定位、核小体滑动和核小体替换等)而改变DNA的“可及性"(accessibility),进而影响特定的生理、生化过程。染色质重塑复合体最早在酵母中发现,生化分析表明其至少含有13个亚基。目前植物染色质重塑复合体的组成还未完全解析,但通过对其酵母同源亚基(染色质重塑因子)的研究可从侧面探究植物染色质重塑复合体的功能。同时,还着重讨论了近年来在植物染色质重塑因子研究上取得的结果,以期为植物染色质重塑的作用机制提供启示。

In eukaryotic cells,the ATP-dependent chromatin remodeling complexes utilize the energy of ATP to disrupt nucleosome DNA contacts,move nucleosomes along DNA,and remove or exchange nucleosomes.They thus make DNA/chromatin available to proteins that need to access DNA or histones directly during cellular processes.The first chromatin remodeling complex was found in yeast,containing at least 11 subunits by biochemical analysis.However,the chromatin remodeling complexes in plants are less known.The studies on plant chromatin remodeling factors were reviewed,which would provide insights into the involvement of plant chromatin remodeling in development.